Ni-NTA Koluma & Papatusi

①Au'ili'ili fa'atauga o oloa

Vaega o oloa: Ni-NTA koluma / ipu mo le polotini peptide antibody faʻataʻitaʻiga sauniuniga ma le faʻamamaina o porotini

Mea: PP + Agarose gel

Volume koluma: 1ml, 3ml, 5ml, 6ml, 12ml ma 2ml 96-pu desalination ma le faamamaina o papatusi

Galuega: fa'atosina vaega o le polotini polypeptide antibody, filtration, adsorption, tete'a, fa'amama, fa'amamāina ma fa'atonuga o fa'ata'ita'iga fa'atatau.

Fa'amatalaga: 0.5ml/1ml, 1ml/3ml, 1.5ml/3ml, 1.5ml/5ml, 2.5ml/5ml, 2ml/6ml, 3ml/6ml, 4ml/12ml, 6ml/12ml, 0.4ml×96, 0.8ml×96

afifiina: 25 fasi / 1ml, 20 fasi / 3ml, 30 fasi / 6ml, 20 fasi / 12ml, 5 fasi / pusa o le koluma chromatography feololo, 1 fasi / pusa o le 96-pu desalination ma le faamamaina ipu.

Mea fa'apipi'i: ato pepa alumini po'o taga pepa alumini fa'amalama (filifiliga)

Pusa afifi: pusa fa'ailoga le tutusa po'o le pusa lanu BML (filifiliga)

logo masani: ioe

auala tuʻuina atu: OEM / ODM

②Fa'amatalaga o oloa

BM olaga faasaienisi Ni-NTA koluma na faamamaina e polotini sootaga chromatography ma agarose e pei o crosslinking medium.O le faamamaina mataupu faavae o le faamamaina o le sini porotini ma tag e ala i le faaaogaina o le meatotino fusifusia faapitoa i le va ions nickel chelated i NTA ma tag.O lenei auala e faigofie e faagaoioia. , tele o le faʻamamaina, faigofie i le faʻamamāina tele o polotini manaʻomia.

Nickel chloride i le koluma Ni e mafai ona fusifusia i le polotini ma lona igoa (histone). A maeʻa ona fusifusia, e mafai ona maua le polotini sini faʻanatura e ala ile imidazole elution.Packing tapulaʻa o Ni koluma i le selau maila le ola faasaienisi.

Su'ega: 4% po'o le 6% agarose feso'ota'i;

Ole lapopoa ole vaega ole fua ole: 90um(45-165um);Fofo teuoloa: 20% vaifofo ethanol;

PH mautu: 2-12;

denaturants tetee: 6M guanidine hydrochloride ma 8M urea;

Taofi le mamafa: <0.3mpa, 0.1mpa ua fautuaina;

Adsorption: >20mg(27kDa) porotini /ml;

Tulaga teuina: 2-8℃.

BM life science Ni preloaded koluma e maua ai oloa o 5 faʻamatalaga, e aofia ai le 1, 3, 5, 6 ma le 12ml. 1ml ma le 5ml o loʻo faʻapipiʻiina muamua koluma o le mamafa o le chromatography, lea e mafai ona faʻaaogaina atoatoa le lelei o le faʻaogaina o le faʻaogaina o le vai mo le vave ma lelei le faʻamamaina ma le faʻamamaina o macromolecules olaola.

③Fa'ailoga oloa:

★fa'amatalaga eseese: 1/3/6/12ml mo tui, 1/5ml mo koluma chromatography feololo;

★maualuga le faapalepale mamafa: chromatography mamafa faʻapipiʻi muamua faʻapipiʻi koluma faʻapalepale mamafa e oʻo atu i le 0.6mpa (6 pa, 87 psi);

★faigofie e faaaoga: ruer interface, e mafai ona faaaoga i le faasologa e faateleina ai le uta faataitai, e mafai foi ona fesootai i tui ma peristaltic pamu, e mafai foi ona fesootai tuusao KTA, Agilent, Shimadzu, Vai ma isi faiga faamamaina vaega vai;

★fa'aaoga lautele: fa'amamā nucleic acid, antibodies, fa'ailoga porotini, fa'a'alaina porotini.

Ofa'amatalaga

Cat Nu. lanu fa'amatalaga fa'amatalaga (ml) fa'apipi'i

PNI001-1 mumu/meamata NI medium pressure chromatography fa'apipi'i muamua koluma 1 5 / pusa

PNI001-2 paipa manino/ufi mumu NI ituaiga tui fa'apipi'i koluma 1 25 / pusa

PNI003-1 pupuni manino/ufi mumu NI ituaiga tui fa'apipi'i muamua koluma 3 20 / pusa

PNI005-1 mumu/lanumeamata NI medium chromatography koluma 5 5 / pusa

PNI006-1 fa'apogai manino/pulou mumu NI ituaiga tui fa'apipi'i muamua koluma 6 30 / pusa

PNI012-1 fa'apogai manino/ufi mumu NI ituaiga tui fa'apipi'i muamua koluma 12 20 / pusa

BM Life Science, o faʻasologa uma o le SPE koluma tubes e faia i le fomaʻi-grade polypropylene tui tui;O le sieve ipu ua sintered ma ultra-mama ultra-maualuga molecular mamafa polyethylene.sorbents faatauina i le lalolagi atoa, ma e ala i iloiloga faatagaina, lelei e faatuatuaina;10,000 vasega mama falea'oga gaosiga, tulaga fa'ata'atia le gaosiga, pulega ERP, tulaga lelei o oloa su'esu'e; fa'amatalaga 'ese'ese oloa e fa'afetaui mana'oga o tagata fa'atau; ina ia mafai ai e tagata faʻatau ona fiafia i le maualuga o le auaunaga e tasi-taofi.

Fa'ailoga o oloa fa'asologa o le BM Life Science SPE

Tulaga fa'apitoa:

★O nisi SPE sorbent suʻesuʻega ma atinaʻe ma gaosiga tekinolosi faʻatasi ai ma aia tatau faʻapitoa tau le atamai, faʻapipiʻiina o le SPE (tufatufaina o le pauta, uta, afifiina) ua faʻaogaina atoatoa.

★Faʻalagolago i tulaga faʻapitoa o le faʻaogaina o le CNC mole inject molding industry i le penina vaitafe delta, o le tuʻufaʻatasia ma le faʻaogaina lelei o punaoa ua faʻaluaina le gaosiga o paipa koluma SPE, faʻaititia le tau o le gaosiga o le gaosiga ma le tui tui, ma faʻaleleia atili ai le gaosiga. tulaga lelei o oloa

★O loʻo i ai i le kamupani se ultrafine tulaga ese i tekinolosi tufatufa paʻu lelei, tufatufaina atu o le pauta atoa i se otometi, batchable, scalable auala e mautinoa ai le vaega o oloa sili atu le mautu

★E fiaselau miliona o R&D ma gaosiga gafatia mo sieve plates ma faamama autu ua faaitiitia le tau o le gaosiga o le SPE i le tulaga maualalo.

★SPE sieve plate e matua tutoʻatasi, o lona lautele, mafiafia, lapoʻa avanoa e mafai ona filifilia ma faʻapipiʻi i le loto.

★le kamupani fa'apipi'i taua tele i le fa'aonaponei fa'atekonolosi ma fa'aauau pea le fa'aleleia atili, aemaise lava Tip SPE, sieves free panel inlaying SPE, 96-384-pu ipu SPE ma aia tatau fa'ale-mafaufau tuto'atasi, lea e fa'atumu ai le avanoa i Saina ma o'o atu i le lalolagi-vasega tulaga, atagia. o tulaga tulaga ese o le B&M Life Science i le SPE fanua.

Tulaga lelei o oloa:

★Fa'afaigofie ona fa'agaoioia, i lalo o le gaioiga o le kalave fa'anatura e mafai ona ausia le tele o le saoasaoa tele ma le toe gaosia lelei

★E mafai ona faʻasaoina le tau o meafaigaluega ma mea faʻaaogaina e aunoa ma le SPE ma masini masini

★mama sorbent e aunoa ma se faalavefau mai avanoa avanoa

★maualuga toe faʻaaogaina fua faatatau o 10 ~ 100ppm sa i le tulaga sili ona lelei o 95% ~ 105%

★Faatasi ai ma le tele o le adsorption gafatia, e sili atu nai lo isi ituaiga koluma SPE i Saina

★tulaga lelei o oloa mautu, toe fa'aleleia lelei, uta fa'atatau o suiga fa'atatau (RSD) <5%

★Le fefe ile koluma mago.Mago ma susu tafe e tutusa i totonu o le mea sese, fesuiaiga tulaga masani (RSD) <0.05%

★o matou oloa e tutusa lelei lava le tulaga lelei e pei o Waters / Agilent / Supelco ma isi kamupani

★O le tau o le fa'atinoga o a tatou oloa o lo'o i totonu o le sili ona lelei i le lalolagi

Talosaga:

★Fa'aaoga lautele i le palapala; Suau'u; Sua o le tino (plasma/urine, etc.); Mea'ai ma isi.

Tulaga lelei:

★Ia mautinoa o oloa uma e agavaa, faʻaaoga tulaga faʻatonutonu lelei, faʻatinoina le asiasiga a le vaega

★Ia mautinoa o oloa taʻitasi e leai se faʻalavelave faʻafuaseʻi, o le fua faatatau o le toe faʻaleleia e sili atu nai lo tulafono a le atunuʻu, e oʻo atu i le maualuga maualuga o oloa tutusa.

Tautoga o faatinoga:

★Tuuina atu le lagolago faʻapitoa faʻapitoa mo le leai o se totogi

Fa'avasegaina ole koluma SPE

Polymer matrix faasologa SPE

Faatasi ai ma le polymer spherical e pei o le adsorbent, o le tele o le sorbent e sili atu le toniga, e sili atu le mautu o le tafe o le koluma SPE, o le koluma polymer masani i le falesuesue, o le faasologa atoa o oloa ua faʻaaogaina lautele i suʻega meaʻai. O oloa e tutusa ma Waters' HLBS, MAX's ma MCX's.

Silica gel matrix faasologa SPE

Classic silica gel matrix SPE koluma, amorphous / spherical adsorbent, faʻatasi ai ma le maualuga o tau faʻatinoga ma le lelei o le faʻaaogaina e le tagata faʻatau oloa. O oloa e faʻaaogaina lautele i suʻesuʻega vailaʻau.

Su'ega fa'amama/fa'amamāina/fa'atotonuga ole SPE

o se tao pipetting masini ma galuega tauave o loʻo faʻaaogaina mo faʻamaʻi / faʻamamāina faʻatamaoaigaina faʻataʻitaʻiga olaola poʻo le Motugaafa o SPE, faʻamoemoega oloa pipetting tao pito i luga faaopoopo nisi C4 / C18 / silicon paʻu / maneta lopa / ProtingA (G) agarose gel sorbent, faʻaaogaina mo primer/genomic DNA plasmid PCR oloa/peptide porotini/antitibodies e fa'atatau i oloa/fa'amama/fa'a'ese'ese/desalting/fa'amamāina/fa'atamaoaigaina.

96/384-pu fa'asologa ipu SPE

O le 96/384-well plate series SPE na atiina ae faʻapitoa mo faʻataʻitaʻiga maualuga faʻataʻitaʻiga muamua.

Fa'asologa fa'apitoa fa'amatalaga SPE

Fa'ailoga fa'ailoga fa'apitoa fa'ailoga: filifili mea fa'atumu diatomite ultra-mama; Fa'atekonolosi fa'atonutonu fa'asolosolo fa'amama fa'amama fa'apitoa, e ala i su'ega fa'alotoifale ma atunu'u i fafo, sapalai OEM.

E le gata i lea, o lo'o i ai graphitized carbon black, acid alkali neutral alumina, activated shell coconut carbon, honey detection column, pa'u decolorization column, plasticizer detection column......SPE column, sili atu oloa SPE faamolemole fesili.

Laasaga (ave le Tip SPE e fai ma faʻataʻitaʻiga):

O le fa'agaioiga e fai si 'ese'ese e fa'atatau i le fa'atumauina o masini sorbent fa'atasi ai ma le adsorption o oloa fa'atatau po'o mea leaga

1. O le sorbent adsorbs le oloa taulaʻi, aveese mea leaga ma eludes le oloa taulaʻi

Fa'avae:

utaina fa'amama fa'ata'ita'iga, fa'ate'aina fufuluina, fa'ase'e

O lenei ituaiga o fa'agaioiga fa'atosina vaega mautu e masani lava ona fa la'asaga:

(1) fa'agaoioia - aveeseina o mea le mama i le Tip SPE ma fa'atupuina o nisi siosiomaga solvent;

(2) uta faʻataʻitaʻiga - faʻamavae le faʻataʻitaʻiga i se mea faʻapitoa, faʻaaoga paipa e manava ai le Tip SPE ma faʻapipiʻi vaega i luga o le Tip;

(3) fa'a'a'a'a - fa'ato'a aveese mea leaga ma isi oloa e le fa'atatau;

(4) elution – elution ma le aoina o le oloa fa'atatau i se vaega itiiti o le solvent.

2. O le sorbent adsorbs le mama ma aveesea le oloa taulai

Fa'avae:

Su'e mea leaga i luga o le fa'ata'ita'iga ma tuli'esea le oloa fa'atatau

O lenei ituaiga o la'asaga malosi e masani ona i ai laasaga e tolu:

(1). Fa'agaoioiga - aveese mea leaga i le Tip SPE ma fa'atupuina se si'osi'omaga fa'afefete.

(2) utaina fa'ata'ita'iga - fa'amama le Tip SPE ile paipa ona feula malie lea i fafo. I le taimi nei, o le tele o mea faʻapipiʻi faʻamoemoe o le a feulaina i fafo ma le faʻataʻitaʻiga faʻavae vaifofo, ma o mea eleelea o le a taofia i luga ole Tip. O le mea lea, o laasaga e tatau ona amata e aoina.

(3) Elution - faʻafefe o vaega i se vaega itiiti o le solvent ma aoina, faʻapipiʻi le suavai aoina.

E sili atu faʻamatalaga poʻo faʻapitoa faʻapitoa, faʻafeiloaʻitagata fou ma tuai uma e fesili, talanoaina felagolagomai, saili atinae masani!