O le fa'asologa lautele mo le su'eina o vaega malosi e fa'apea:

1. Fa'aagaoioia le adsorbent: Fa'amama le fa'amalosi vaega fa'apipi'i fa'atasi ma se solvent talafeagai a'o le'i aveese le fa'ata'ita'iga e fa'asusu ai le adsorbent, lea e mafai ona fa'afefeteina fa'apipi'i po'o fa'alavelave fa'alavelave. O auala eseese o le fa'agaoioia o kesi fa'atosina vaega malosi e fa'aogaina ai solvents eseese:

(1) O mea fa'apipi'i vaivai po'o mea e le-polar o lo'o fa'aaogaina i le fa'aliliuina o le la'au fa'ato'aga e masani ona fa'amama i se vai solu solu vai, pei o le methanol, ona fufulu lea i le vai po'o se vaifofo pa'u. E mafai fo'i ona fa'amama i se so'o malosi (e pei o le hexane) a'o le'i fufulu ile methanol e fa'aumatia ai mea leaga o lo'o fa'apipi'i ile adsorbent ma lo latou fa'alavelave i le fa'apipi'i fa'atatau.

(2) O le fa'apipi'i polar o lo'o fa'aogaina i le fa'aputuga fa'a-malō-fa'asao masani e masani ona fa'afefeteina ma le so'o fa'aola (sample matrix) lea o lo'o i ai le fa'aputuga fa'atatau.

(3) O le adsorbent o loʻo faʻaaogaina i le faʻaogaina o le faʻaogaina o le ion-exchange solid phase e mafai ona fufulu faʻatasi ma le solvent faʻataʻitaʻiga pe a faʻaaogaina mo faʻataʻitaʻiga i solvents organic non-polar; pe a faʻaaogaina mo faʻataʻitaʻiga i solvents polar, e mafai ona fufulu i vai soluble organic solvents A maeʻa le fufulu, fufulu i se vaifofo o le pH talafeagai ma o loʻo i ai nisi solvents organic ma masima.

Ina ia mafai ona faʻasusu le sorbent i totonu o le pusa SPE pe a uma ona faʻagaoioia ma aʻo leʻi faʻaopoopoina faʻataʻitaʻiga, e tusa ma le 1 ml o le solvent mo le faʻagaoioia e tatau ona teuina i luga o le sorbent pe a uma ona faʻagaoioia.

2. Fa'ata'ita'iga fa'amomoli: Liligi le vai po'o le fa'ata'ita'iga ma'a'a fa'ata'ape'apeina i totonu o le fa'ato'a fa'agaoioi vaega malo, ona fa'aoga lea o le gaogao, mamafa po'o le centrifugation e fa'aulu ai le fa'ata'ita'iga ile adsorbent.

3. Fufulu ma elution: A maeʻa ona ulufale le faʻataʻitaʻiga i totonu o le adsorbent ma faʻapipiʻi le faʻapipiʻi faʻapipiʻi, e mafai ona fufulu ese le faʻalavelave faʻafefe vaivai i se solvent vaivai, ona mafai lea ona faʻafefeteina le faʻapipiʻi ma se solvent malosi ma aoina. . Fufulu ma Elution E pei ona faamatalaina muamua, o le eluent po'o le eluent e mafai ona pasia i totonu o le adsorbent e ala i le gaogao, mamafa poʻo le centrifugation.

Afai e filifilia le adsorbent e vaivai pe leai se adsorption i le faʻapipiʻi faʻapipiʻi ma faʻamalosi malosi i le faʻalavelave faʻalavelave, e mafai foi ona fufulu ma aoina muamua le mea faʻapipiʻi, aʻo taofia le faʻalavelave faʻalavelave (adsorption). ) i luga o le adsorbent, o le lua ua tuueseeseina. I le tele o tulaga, o le faʻapipiʻiina o loʻo faʻamauina i luga o le adsorbent, ma mulimuli ane faʻafefeteina ma se solvent malosi, lea e sili atu ona faʻaogaina i le faʻamamaina o le faʻataʻitaʻiga.