QuECHERS (Byihuse, Byoroshye, kuba bihendutse, Bikora neza, Byangiritse kandi bifite umutekano) byateye imbere mumahanga mumyaka yashize byateje imbere uburyo bwihuse bwo gutegura icyitegererezo cyihuse cyo kumenya ibicuruzwa byubuhinzi. Yatunganijwe bwa mbere na M Anastassisdes wo mu ishami ry’ubuhinzi muri Amerika (2003), nyuma yo kugenzura no kunoza byinshi, uburyo bwa QuEChERS bwashyizwe ahagaragara. Uburyo bwa QuEChERS bwo gutegura ibisigazwa byica udukoko twangiza udukoko twangiza, birasa ningaruka zo kweza SPE, ariko intambwe yo gutunganya irasobanutse neza, ifite ibiranga guta igihe, gukora neza, ubukungu kandi byemerwa buhoro buhoro nisesengura rusange ryabakozi. Ukoresheje ibikoresho bya QuEChERS, gusa intambwe nke zoroshye zirasabwa kugirango urangize icyitegererezo cyo gutegura ibyiciro byinshi hamwe nisesengura ryica udukoko twinshi.

Ibicuruzwa bya BM - Q nintambwe nziza yubumenyi bwa Shenzhen BM Ubuzima bushingiye ku iterambere ryibicuruzwa bitunganyirizwa byihuse bya QuEChERS, urutonde rwicyitegererezo rushobora gukoreshwa mugutahura ibisigazwa byica udukoko, gutahura ibisigazwa byibiyobyabwenge byamatungo, inyongeramusaruro yibiribwa, gutahura nibindi bice. Ubu bwoko bwibicuruzwa burashobora gukoreshwa muburyo bwinshi, butandukanye bwa adsorbent, nka BM - GCB depigmentation, BM - PSA kugirango ikureho proteyine na acide organic, BM - NH2 ikuraho aside organic, BM - WCX isukura ibintu bya alkaline, BM - C18 kura ibinure nibindi bintu kama, alumina ikureho ibinure, anhidrous magnesium sulfate ikuraho ubuhehere. Byongeye kandi, wongeyeho umunyu wa buffer, birashobora gufasha gukuramo neza imiti.Uburyo butandukanye bwo gukusanya bushobora kuba yemejwe kugirango ihuze n'ibikenewe bitandukanye.

Ibiranga urutonde rwa BM-Q:
★Igipimo kinini cyo gukira ibiyobyabwenge:igipimo gishimishije cyo gukira hafi ya pesticide nyinshi.
★Urutonde runini rwa porogaramu:yo gusesengura ingero zitandukanye.
★Inzira yoroshye:uburyo buroroshye, intambwe yo gukora ni nto, kandi ingaruka zibintu byabantu kumiterere yuburyo burashobora kugabanuka, mugihe imikorere ya laboratoire ishobora kunozwa kandi imikorere ya laboratoire ikanozwa.
★Ibisubizo byizewe:ibikoresho fatizo fatizo biva mubitanga bimwe, kandi guhuzagurika no gutuza kwintego birizewe.
★Kurengera ibidukikije:ikoreshwa rya solvent ni rito, kurengera ibidukikije bisukuye, nyuma yo kongeramo ibishishwa, birashobora guhita bifunga kontineri, kugabanya ibyangiritse kubakoresha.

Ibisobanuro:

Icyitegererezo:

Kugirango hamenyekane uburinganire bw'icyitegererezo. Mbere yo gukoresha, ingero zikomeye nk'imbuto n'imboga zigomba gutemwa kandi zigahuzwa, kandi icyitegererezo cy'imboga n'imbuto ubusanzwe ni 10-15g, hamwe no guhuza imashini yihuta cyane ya mashini (RPM 11000) r / min-24000 r / min).

Intambwe ya 1: gukuramo icyitegererezo:

Ingano yapimwe yicyitegererezo yamenetse iri mumiyoboro ya BM-Q ikuramo, yongeramo ibishishwa bikenerwa mumashanyarazi ya BM-Q, kandi gukuramo homogenizing cyangwa ultrasonic byakoreshejwe mugusukura amazi.

Intambwe ya 2: kwezwa

Ukurikije icyitegererezo, hitamo umuyoboro ukwiye wa BM-Q usukuye, hanyuma wongeremo ibivuye mumiyoboro isukuye, uzunguze neza hanyuma uvange neza, hanyuma ushireho centrifuge yibitekerezo byamazi cyangwa gutahura icyitegererezo. Nyuma yo kweza umuyoboro usukuye, matrix igoye irashobora komeza usukure amazi yisuku kugirango ugere kubintu byiza byo kweza.

Inyandiko:

Iyo icyitegererezo cyahujwe n'umurongo wa purge, sulfate ya anhidrous magnesium sulfate irashobora kugira umuriro na gaze, Ibyifuzo byumuvuduko ukabije nyuma yigihe runaka nyuma yo gufungura umuyoboro wa centrifuge ugana kunyeganyega hamwe nibindi bikorwa.

Iyo amazi arimo amazi menshi yongewe kumuyoboro usukuye urimo sulfate ya magnesium, nyamuneka ongeramo umusemburo mbere yo kongeramo icyitegererezo kugirango wirinde ibisubizo bya cake ya sample na sulfate ya magnesium.

Nyamuneka nyamuneka witondere gufunga ibicuruzwa.Iyo ibicuruzwa bipakiye, birashobora gufungwa mumufuka ufunze cyangwa bikabikwa ahantu humye cyangwa bihumeka.

Ibibazo bihari muburyo bwambere QuEChERS:

Muri acide iciriritse (orange), ugereranije ugereranije na alkaline yica udukoko twica udukoko twinshi; Ndetse no muri matrike itabogamye, imiti yica udukoko twangiza alkaline.

Ibisubizo:

Muburyo bwo gusesengura acide acide (orange) kugirango yinjire mu mvange ya HAC na NaAc ivanze, irashobora gukomeza sisitemu yo gukemura pH hagati ya 4 kugeza 5, kugirango bikemure ikibazo cyo gukira kwica udukoko twangiza alkaline.

BM-ABABAZI Tegeka amakuru
     Uburyo bw'icyitegererezo cyo gutegura, bwatanzwe na Anastassiades mu 2003, bwakoreshejwe cyane mu gutahura ibiribwa, amazi y’ibidukikije ndetse n’ibyanduza ubutaka. Ibicuruzwa by’isosiyete yacu bireba ishami ry’ubuhinzi mu Bushinwa, NY / T, AOAC na eu EN.

Uru ruhererekane rwibicuruzwa rushobora kumenya byimazeyo gukwirakwiza, gupakira no gupakira ifu, kumenya kwikora, igipimo n’umusaruro rusange, kandi ukemera kugenwa byihariye.

Ikaze abakiriya bashya kandi bashaje kubaza, kuganira kubufatanye no gushaka iterambere rusange!