Nubuhe buryo bwo kweza bwasisitemu yo kweza poroteyine? Birakenewe kumenya urutonde rwa ADN ikurikirana ya poroteyine isukuye, kugirango turebe ingirabuzimafatizo cyangwa ingirabuzimafatizo zikabije muri gene igenewe, no gukora gene primers kugirango yongere arf igice cya ADN igenewe. Nicyo bita kugura intego ya gene ibice.

Kubaka imvugo yerekana: Muri prokaryotic cyangwa eukaryotic imvugo ya vector hamwe nibiranga imvugo ya gene ubwayo ubwayo, ikibazo nyamukuru cyiyi ntambwe nukubaka plasmid na gene yinyungu, hamwe na sisitemu yo kwerekana. Imvugo ya prokaryotic ni ngufi, ikiguzi ni gito, kandi umubare munini wo kuvuga niwo wambere; gene ntigaragarira muri E. coli, kandi ikibazo kibaho muburyo bwiza bwa codon. Urebye ibikorwa byiza no kweza poroteyine, abashakashatsi bahisemo kwerekana mu musemburo wa Pichi. Kugaragaza neza uburyo bwiza bwa codon ni ngombwa.

Nubuhe buryo bwo kweza sisitemu yo kweza poroteyine:

1. Imvura.

2. Shyigikira firime electrophoreis, electrophoreis, nibindi.

3. Dialysis: Uburyo bukoresha imifuka ibiri ya dialyse kugirango itandukanye molekile nini na poroteyine hamwe n’ibinyabuzima bito bito.

4. Chromatografiya: Ion guhana chromatografiya ikoresha ibintu byubusa bya poroteyine. Munsi ya pH yihariye, ibirego nibintu bya poroteyine biratandukanye, kandi birashobora gutandukanywa na ion guhana chromatografiya. Muri anion guhana chromatografiya, proteyine zifite imbaraga nkeya zitangwa mbere. Amashanyarazi ya molekuline, azwi kandi nka filtration ya gel. Poroteyine nto zinjira mu byobo kandi zigumamo igihe kirekire. Poroteyine nini ntizishobora kwinjira mu myobo kandi zisohoka mu buryo butaziguye.

5. Ni ubuhe buryo bwo kweza bwasisitemu yo kweza poroteyine? Ultracentrifugation: Isuku ya poroteyine irashobora gukoreshwa kugirango umenye uburemere bwa molekile kandi irashobora gukoreshwa nka poroteyine. Ihinduka rya poroteyine zifite ubucucike butandukanye ziratandukanye.