Ihame ryibanze nibiranga aside nucleic ikuramo

Sisitemu ya Nucleic Acide Sisitemu (Nucleic Acide Extraction Sisitemu) ni igikoresho gikoresha reagent yo gukuramo aside nucleic kugirango ihite yuzuza icyitegererezo cya acide nucleic. Ikoreshwa cyane mu bigo bishinzwe kurwanya indwara, gusuzuma indwara z’amavuriro, umutekano wo guterwa amaraso, kumenyekanisha ubutabera, gupima mikorobe y’ibidukikije, gupima umutekano w’ibiribwa, ubworozi n’ubushakashatsi bw’ibinyabuzima bya molekuline n’izindi nzego.
1. Uburyo bwo guswera, buzwi kandi nk'uburyo bwo kuvoma, ni ugukuramo aside nucleic mu guhagarika amasaro ya magneti no kohereza amazi. Mubisanzwe, ihererekanyabubasha rikorwa mugucunga ukuboko kwa robo binyuze muri sisitemu y'imikorere. Igikorwa cyo kuvoma nuburyo bukurikira:

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1) Lysis: Ongeramo igisubizo cya lysis kurugero, hanyuma umenye kuvanga hamwe nigisubizo cyuzuye cyigisubizo cya reaction binyuze mumashanyarazi no gushyushya, selile ziraterwa, hanyuma aside nucleique irekurwa.

) Munsi yibikorwa byumwanya wo hanze, amasaro ya magneti yatandukanijwe numuti. , koresha inama kugirango ukureho amazi hanyuma uyijugunye mumyanda, hanyuma ujugunye hejuru.

3) Gukaraba: Kuraho umurima wa magneti wo hanze, usimbuze inama nshya hanyuma wongereho gukaraba, kuvanga neza kugirango ukureho umwanda, kandi ukureho amazi munsi yumurimo wa magneti yo hanze.

4) Kurandura: Kuraho umurima wa magnetiki wo hanze, usimbuze inama nshya, ongeramo buffer ya elution, uvange neza, hanyuma utandukanye aside nucleic ihambiriye kumasaro ya magneti kugirango ubone aside nucleique isukuye.
2. Uburyo bwa magneti

Uburyo bwa magnetiki inkoni itahura gutandukanya acide nucleic mugukosora amazi no guhererekanya amasaro ya magneti. Ihame nibikorwa birasa nuburyo bwo guswera, ariko itandukaniro nuburyo bwo gutandukanya amasaro ya magneti namazi. Uburyo bwa magnetiki bar ni ugutandukanya amasaro ya magneti n'amazi yimyanda binyuze mumyanya ya adsorption yinkoni ya magneti kugeza kumasaro ya magneti, hanyuma ukayashyira mumazi akurikira kugirango umenye gukuramo aside nucleique.


Igihe cyo kohereza: Gicurasi-24-2022