Uburyo bukomeye bwa microextraction uburyo

SPME ifite ibyingenzi bitatugukuramouburyo: Direct Ectraction SPME, Umwanya Umwanya SPME na SPME irinzwe na membrane.

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1) Gukuramo mu buryo butaziguye

Muburyo bwo gukuramo butaziguye, fibre ya quartz yashizwemo nagukuramoIcyiciro gihagaze cyinjijwe muburyo butaziguye matrike, kandi intego zigenewe kwimurwa biturutse kuri sample ya matrix ikuramo icyiciro gihagaze. Mugihe cyibikorwa bya laboratoire, uburyo bwo guhagarika umutima bukoreshwa muburyo bwihuse bwo gukwirakwiza ibice byisesengura kuva matrike y'icyitegererezo kugeza ku nkombe yo gukuramo icyiciro gihagaze. Kuburugero rwa gaze, convection isanzwe ya gaze irahagije kugirango yihutishe kuringaniza ibice bigize isesengura hagati yibyiciro byombi. Ariko kuburugero rwamazi, umuvuduko wo gukwirakwiza ibice mumazi ni ordre 3-4 yubunini buri munsi ugereranije na gaze, bityo hakenewe tekinoroji yo kuvanga neza kugirango habeho ikwirakwizwa ryihuse ryibigize murugero. Uburyo bukoreshwa cyane bwo kuvanga harimo: kwihutisha umuvuduko wikitegererezo, kunyeganyeza fibre fibre umutwe cyangwa ibikoresho byabigenewe, rotor ikurura na ultrasound.

Ku ruhande rumwe, ubwo buhanga bwo kuvanga bwihutisha ikwirakwizwa ryibigize muri matrike nini yintangarugero, naho kurundi ruhande, bigabanye ingaruka bita "igihombo cya zone" iterwa nigice cyamafirime yo gukingira firime yakozwe kuri urukuta rwo hanze rwo gukuramo icyiciro gihagaze.

2) Gukuramo Umwanya

Muburyo bwo gukuramo umutwe, inzira yo gukuramo irashobora kugabanywamo intambwe ebyiri:
1. Ibice byasesenguwe biratandukana kandi byinjira kuva mugice cyamazi kugeza icyiciro cya gaze;
2. Igice cyasesenguwe cyimurwa kiva mucyiciro cya gaze kijya gukuramo icyiciro gihagaze.
Iri hinduka rirashobora gukumira icyiciro cyo gukuramo icyiciro cyanduye kwanduzwa nibintu byinshi bya molekile nyinshi hamwe n’ibintu bidahindagurika muri matrice zimwe na zimwe z'icyitegererezo (nk'ururenda rw'abantu cyangwa inkari). Muri ubu buryo bwo kuvoma, umuvuduko wo gukuramo intambwe ya 2 muri rusange uruta cyane umuvuduko wo gukwirakwiza intambwe ya 1, bityo intambwe ya 1 ihinduka intambwe yo kugenzura. Kubwibyo, ibice bihindagurika bifite igipimo cyihuta cyo gukuramo kuruta igice cya kabiri gihindagurika. Mubyukuri, kubice bihindagurika, mugihe kimwe cyo kuvanga ibintu, igihe cyo kuringaniza umwanya wo gukuramo umutwe ni kigufi cyane ugereranije no gukuramo bitaziguye.

3) Gukuramo Membrane

Intego nyamukuru yo kurinda membrane SPME ni ukurindagukuramoicyiciro gihagaze kuva kwangirika mugihe usesenguye ingero zanduye cyane. Ugereranije no gukuramo umutwe SPME, ubu buryo nibyiza cyane mugukuramo no gutunganyiriza ibintu bigoye-guhindagurika. Mubyongeyeho, firime ikingira ikozwe mubikoresho bidasanzwe itanga urwego runaka rwo guhitamo inzira yo gukuramo.


Igihe cyo kohereza: Apr-07-2021