Gutandukanya no kweza poroteyine bikoreshwa cyane mubushakashatsi bwibinyabuzima no kubukoresha kandi ni ubuhanga bukomeye bwo gukora. SCG Protein Sisitemu Sisitemu-Saipu Igikoresho cyegeranije itandukaniro rito hamwe nibitandukanya byiza byaporoteyinekwezwa kuri buri wese. Ingirabuzimafatizo ya eukaryotique irashobora kuba irimo poroteyine ibihumbi n'ibihumbi, zimwe zirakize cyane kandi zirimo kopi nkeya. Kugirango wige poroteyine runaka, ni ngombwa kubanza kweza poroteyine mu zindi poroteyine na molekile zitari poroteyine.

Gutandukana gukabije

Iyo habonetse poroteyine (rimwe na rimwe ivangwa na acide nucleic, polysaccharide, nibindi) ibonetse, hashyizweho uburyo bukwiye bwo gutandukanya ibyifuzo.poroteyinebiturutse ku bindi byanduye. Mubisanzwe, iyi ntambwe yo gutandukana ikoresha uburyo nko gusohora, gukusanya ingingo ya isoelectric hamwe no gucamo ibice kama. Ubu buryo burangwa nubworoherane nubushobozi bunini bwo gutunganya, bushobora gukuraho umwanda mwinshi no kwibanda kuri proteine. Ibikomoka kuri poroteyine bimwe na bimwe ni binini kandi ntibikwiriye kwibanda ku kwegeranya cyangwa gusohora. Urashobora guhitamo ultrafiltration, gel filtration, gukonjesha vacuum cyangwa ubundi buryo bwo kwibanda.

Gutandukana neza

Nyuma yo gucikamo ibice by'icyitegererezo, ingano muri rusange ni nto, kandi imyanda myinshi yakuweho. Kugirango urusheho kwezwa, uburyo bwa chromatografiya burimo gushungura gel, gushiramo ion chromatografiya, adsorption chromatografiya, hamwe na chromatografi ya affinity. Nibiba ngombwa, urashobora kandi guhitamo electrophoreis, harimo zone electrophoreis, point ya isoelectric point set, nibindi nkibikorwa byanyuma byo kwezwa. Uburyo bwakoreshejwe murwego rwo kugabana gutandukana mubisanzwe ni bito mugutegura, ariko hamwe nibisubizo bihanitse.

Crystallisation ninzira yanyuma yo gutandukanya poroteyine no kwezwa. Nubwo uburyo bwo gutegera butemeza ko poroteyine igomba kuba imwe, ni mugihe poroteyine runaka ifite inyungu mugisubizo cyo gukora kristu. Inzira yo korohereza ubwayo iherekejwe n’urwego runaka rwo kwezwa, kandi kongera gukora ibintu bishobora gukuraho poroteyine nkeya. Kuva yatandukanaporoteyinentabwo yigeze iboneka mugihe cyo gutegera, korohereza poroteyine ntabwo ari ikimenyetso cyera gusa, ahubwo ni umurongo ngenderwaho ukomeye wo kumenya ko ibicuruzwa biri mumiterere yabyo.