Ibicuruzwa Byacu Bishyushye-Ultrafiltration Centrifugal Tube (Centrifugal Filter) Ibicuruzwa bikurikirana

------ "Ultrafiltration & Microfiltration" Intego nyinshi, kumenya gusimburana murugo!

Ubuzima bwa BMUltrafiltration Centrifugal Tube (Akayunguruzo ka Centrifugal)Ibicuruzwa bikurikirana

Igizwe n'umuyoboro w'imbere (hamwe na membrane) + umuyoboro wo hanze wa centrifuge, ushobora kweza vuba kandi neza kandi ukagera kuri 15ml by'ibinyabuzima. Igishushanyo cyihariye cya vertical hamwe nubuso bunini bwo kuyungurura birashobora gutanga ubushobozi bwihuse bwo gutunganya hamwe nigipimo cyinshi cyo kugarura ibintu (mubisanzwe ≥90% byoroheje igisubizo cyumwimerere), mugihe gikomeza gushungura byoroheje hamwe nibidukikije kugirango bikomeze ibikorwa bya poroteyine no Guhinduka. Igishushanyo cyihariye gishobora guhindagurika. igisubizo kandi kigabanya cyane ikosa rya filteri membrane iterwa na centrifugation ikurikira. Muguhindura uburyo bwa filteri yumubiri ihagarara mugikoresho cyo kuyungurura, irashobora kubuza gushungura centrifugal kumisha icyitegererezo cyangwa gutera igihombo cyicyitegererezo kubera kuzenguruka cyane. . Ibice bitandukanye bya ultrafiltration ya molekuline yuburemere (MWCO) ya santrifugali ikoresha filtre ikoresha ubuziranenge bwinshi, adsorption nkeya PES na RCE membrane, ifite proteine ​​nkeya cyane hamwe na aside nucleic aside ihuza.

BM Ubuzima Ubumenyi, ibyumba bisukuye mubyiciro byose byaUltrafiltration Centrifugal Tube (Akayunguruzo ka Centrifugal), imikorere yumurongo, imikorere ya ERP yuzuye, ibicuruzwa birenze urugero, nta DNase / RNase, nta PCR ibuza, nta soko yubushyuhe. Ubuzima bwa BM Ubumenyi, ingano zose zaUltrafiltration Centrifugal Tube (Akayunguruzo ka Centrifugal)Ibicuruzwa byuruhererekane birateguwe, urukurikirane rwibicuruzwa ruhagaze neza hamwe nibitandukaniro rito hagati yicyiciro kandi murwego rwo hejuru. Bashobora gusimburwa n’ibicuruzwa byo mu gihugu no koherezwa mu Buyapani, Koreya yepfo, Uburayi, na Amerika. Byakoreshejwe cyane mubisanzwe icyitegererezo cyo gutandukanya no gushungura.

Gusaba

1 UltrafiltrationGusimbuza Buffer, kweza, kwibanda, no gusibanganya antigene, antibodies, enzymes, nizindi poroteyine, hamwe nubushakashatsi bwibinyabuzima nka acide nucleic acide na mikorobe, kuvanaho ibice bya chromatografique, gutandukanya molekile yibinyabuzima n'umuco wa tissue cyangwa ibisubizo bya lysis selile, kuvanaho ya primers, umuhuza, cyangwa ibimenyetso bya molekuline bivuye kuvanga reaction, kwibanda kuri virusi cyangwa gutandukana, no gutandukanya byimazeyo ivangwa rya molekile ya biologiya.

2 Microfiltrationitandukanya acide nucleic na proteyine muri geles, ingero zirayungurura kandi zirasobanurwa, kandi ibice bitunganijwe cyangwa amasaro byegeranijwe bikaraba.

Ikiranga ibicuruzwa

Ikigereranyo cyo hejuru cyane, gishobora kugera ku nshuro 80-100 igipimo cyo kwibanda ;

Icyitegererezo cyo kwibandaho cyihuta, kandi igihe cyo kwibandaho ni iminota 10-60. Ifite kandi anti-yumye igishushanyo mbonera cyo kwirinda kwangirika kwicyitegererezo kubera centrifugation ikabije ;

Rate Icyitegererezo cyo gukira ni kinini kandi gishobora kugera ku gipimo cyo gukira kirenga 90% ;

Ad adsorption nkeya, PES / RC membrane hamwe nurukuta rwimbere rwimbere rufite poroteyine nkeya cyane hamwe na biomolecule ihuza igipimo ;

4ml / 15ml 、 15ml / 50ml

Ibisobanuro byuzuye, ultrafiltration centrifuge tubes ifite umubumbe wa 4ml / 15ml na 15ml / 50ml, hamwe nubunini butandukanye bwa pore, iboneka muri 3KD, 5KD, 10KD, 30KD, 50KD na 100KD kugirango wuzuze ibisabwa bitandukanye byo kugabanya ibiro bya molekile ;

Operation Ibikorwa byoroshye, gutandukana kwose hamwe no kuyungurura birashobora kurangizwa hamwe naCentrifuge, byoroshye gukora ;

Ibicuruzwa bifite isuku, amahuza yose akorerwa mucyumba gisukuye, imikorere yumurongo winteko, imiyoborere yuzuye ya ERP, ibicuruzwa ni ultra-yera, nta DNase / RNase, nta PCR ibuza, nta soko yubushyuhe;

Definition Ibisobanuro bihanitse, nta background na auto-fluorescence, kandi ntabwo bigira ingaruka kubisubizo;

Performance Imikorere yizewe, gufunga neza, kandi ntigire icyo ikora hamwe nubushakashatsi bwakozwe, ibisubizo byubushakashatsi byizewe, kandi birashobora kwihanganira ubushyuhe bwo hejuru bwa 100 ℃!

★ Ifite intera nini ya porogaramu. Kuri Porogaramu zitandukanye, ubwoko bubiri bwaUltrafiltration Centrifugal Tube (Akayunguruzo ka Centrifugal)ibicuruzwa bikurikirana, 4ml / 15ml na 15ml / 50ml byamenyekanye kugirango bikemure abakiriya batandukanye, bemera kwihitiramo no guteza imbere imikorere!

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Tegeka amakuru

Injangwe. Oya Izina Ibisobanuro Ibisobanuro Pcs / pk
UCT015RCE004003 Ultrafiltration Centrifugal Tube 4 / 15ml , 3kD , RCE Membrane 15 pc / igikapu
UCT015RCE004010 Ultrafiltration Centrifugal Tube 4 / 15ml , 10kD RCE Membrane 15 pc / igikapu
UCT015RCE004030 Ultrafiltration Centrifugal Tube 4 / 15ml , 30kD RCE Membrane 15 pc / igikapu
UCT015RCE004100 Ultrafiltration Centrifugal Tube 4 / 15ml , 100kD RCE Membrane 15 pc / igikapu
UCT050RCE015003 Ultrafiltration Centrifugal Tube 15 / 50ml , 3kD RCE Membrane 24 pc / igikapu
UCT050RCE015010 Ultrafiltration Centrifugal Tube 15 / 50ml , 10kD RCE Membrane 24 pc / igikapu
UCT050RCE015030 Ultrafiltration Centrifugal Tube 15 / 50ml , 30kD RCE Membrane 24 pc / igikapu
UCT050RCE015100 Ultrafiltration Centrifugal Tube 15 / 50ml , 100kD RCE Membrane 24 pc / igikapu
UCT050PES015005 Ultrafiltration Centrifugal Tube 15 / 50ml , 5kD PES Membrane 12 pc / igikapu
UCT050PES015010 Ultrafiltration Centrifugal Tube 15 / 50ml , 10kD PES Membrane 12 pc / igikapu
UCT050PES015030 Ultrafiltration Centrifugal Tube 15 / 50ml , 30kD PES Membrane 12 pc / igikapu
UCT050PES015050 Ultrafiltration Centrifugal Tube 15 / 50ml , 50kD PES Membrane 12 pc / igikapu
UCT050PES015100 Ultrafiltration Centrifugal Tube 15 / 50ml , 100kD PES Membrane 12 pc / igikapu
CF050PES015C010 Akayunguruzo 15 / 50ml , 0.1um 0.1um , PES Membrane 12 pc / igikapu
CF050PES015C020 Akayunguruzo 15 / 50ml , 0.2um 0.2um , PES Membrane 12 pc / igikapu
CF050PES015C045 Akayunguruzo 15 / 50ml , 0.45um 0.45um , PES Membrane 12 pc / igikapu
UCT ** Ultrafiltration Centrifugal Tube Guhitamo Guhitamo ** pcs / igikapu
CF ** Akayunguruzo Guhitamo Guhitamo ** pcs / igikapu

Ubuzima bwa BM, Ultrafiltration Centrifugal Tube (Centrifugal Filter) ibipimo byibicuruzwa hamwe namakuru yubushakashatsi

Ultrafiltration Centrifugal Tube (Centrifugal Filter) ikurikirana ryibicuruzwa hamwe namakuru yubushakashatsi

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avsdv (9)

Ultrafiltration Centrifugal yintangarugero:

5 KD 0.25mg / ml Cyt C ;

10 KD1mg / ml OVA ;

30/50 KD 1mg / ml BSA ;

100 KD 1mg / ml IgG。

Imiterere yubushakashatsi: rotor ya horizontal, imbaraga za centrifugal 4000 × g, 8 ℃, gutangira ingano 15ml.

Ultrafiltration Centrifugal yintangarugero:

5 KD 0.25mg / ml Cyt C ;

10 KD 1mg / ml OVA ;

30/50 KD 1mg / ml BSA ;

100 KD 1mg / ml IgG.

Imiterere yubushakashatsi: inguni ihamye, imbaraga za centrifugal 5000 × g, 8 ℃, gutangira ubunini bwa 12ml.

Igipimo gisanzwe cyo kwangwa kubipimo byuburemere bwa poroteyine (4000g x 15ml ingano yo gutangira rotor cyangwa 5000g x 12ml yo gutangira ingano ya rotor ihamye)

ibipimo by'uburemere bwa molekuline / kwibanda uburemere bwa molekile NMWL Ultrafiltration tube igipimo cyo kwangwa% Shiraho indobo Igipimo cyo kwangwa% inguni ihamye igihe cya centrifugation
B-12 vitamine 1350 3K < 25 < 25 60
(0.25mg / ml) Cytochrome C. 12400   > 95 > 95 60
(1mg / ml) α-Chymotrypsinogen 25000   > 95 > 95 60
B-12 (0.2mg / ml) vitamine 1350 10K < 5 < 5 30
0,25mg / ml) Cytochrome C. 12400   > 95 > 95 30
(1mg / ml) α-Chymotrypsinogen 25000   > 95 > 95 30
vitamine B-12 (0.2mg / ml) 1350 30K < 5 < 5 20
Cytochrome C 0.25mg / ml) 12400   < 10 < 10 20
Ovalbumin (1mg / ml) 45000   > 95 > 95 20
BSA (1mg / ml) 67000   > 95 > 95 20
Cytochrome C (0,25mg / ml) 12400 50K < 5 < 5 10
Ovalbumin (1mg / ml) 45000   65 55 10
BSA (1mg / ml) 67000   > 90 > 90 10
Ovalbumin (1mg / ml 45000 100K < 25 < 15 20
IgG (1mg / ml) 156000   > 90 > 90 20
Thyroglobuline (0.5mg / ml) 677000   > 90 > 90 20

Igipimo cyo gukira

Ubushobozi bwo kugumana 15ml ultrafiltration centrifuge tube membrane isobanurwa no kugabanya uburemere bwa molekile (MWCO).

Ibipimo bya molekuline byuzuye hafi ya MWCO birashobora kugumana igice gusa. Kugirango ubone ibyiza byo kugumana,

nyamuneka koresha MWCO muyunguruzi ya membrane ifite uburemere bwa 1/3 cyangwa munsi ya solute.

Kugereranya igipimo cyo kugarura ibicuruzwa

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Kugereranya ibipimo byo kugarura poroteyine nubunini bwapfuye bwibirango bitandukanye bya ultrafiltration tubes

10KD Kugereranya ibipimo byo kugarura poroteyine kandi byapfuye

ingano yibirango bitandukanye bya ultrafiltration tubes

Ikirango (% Rate Igipimo cyo gukira (Μl volume Ingano yapfuye
BM 1㎎ / ml Intungamubiri za poroteyine 90 87 concent Intungamubiri za poroteyine1㎎ / ml)
0.1㎎ / ml Intungamubiri za poroteyine 83  
Ikirango M. 1㎎ / ml Intungamubiri za poroteyine 93 47 concent Intungamubiri za poroteyine1㎎ / ml)
  0.1㎎ / ml Intungamubiri za poroteyine 88  
Ikirango S. 1㎎ / ml Intungamubiri za poroteyine 91 70 concent Intungamubiri za poroteyine1㎎ / ml)
  0.1㎎ / ml Intungamubiri za poroteyine 85  
Ikirango K. 1㎎ / ml Intungamubiri za poroteyine 89 186 concent Kwibanda kuri poroteyine1㎎ / ml)
  0.1㎎ / ml Intungamubiri za poroteyine 77  
Ikirango J. 1㎎ / ml Intungamubiri za poroteyine 83  
  0.1㎎ / ml Intungamubiri za poroteyine 50  
  Imiterere yubushakashatsi: poroteyine ya OVA (uburemere bwa molekile 45KD), rotor ya horizontal 4000g
 

Ibyatoranijwe guhitamo ibisobanuro bya ultrafiltration tubes ifite uburemere butandukanye bwa molekile

  ingano yo gutangira < 0.5ml < 2ml < 4ml < 15ml
Poroteyine
(MW) uburemere bwa molekile   MWCO (Da) kugabanya uburemere bwa molekile      
  6K < MW < 20K 3000 3000 3000 3000
  20K < MW < 60K 10000 10000 10000 10000
  60K < MW < 100K 30000 30000 30000 30000
  100K < MW < 200K 50000 50000 50000 50000
  200K < MW 100000 100000 100000 100000
Acide nucleic-imwe imwe hamwe na acide nucleic acide
uburebure   MWCO (Da) kugabanya uburemere bwa molekile      
  137-1159bp 3000 3000 3000 3000
nanoparticles
IA DIA size ingano yubunini   MWCO (Da) kugabanya uburemere bwa molekile      
  1.5 < DIA < 3nm 3000 3000 3000 3000
  3 < DIA < 5nm 10000 10000 10000 10000
  5 < DIA < 7nm 30000 30000 30000 30000
  7 < DIA < 10nm 50000 50000 50000 50000
  10 nm < DIA 100000 100000 100000 100000

Igihe cyo kohereza: Gashyantare-18-2024