------ "Ultrafiltration & Microfiltration" Intego nyinshi, kumenya gusimburana murugo!
Ubuzima bwa BM,Ultrafiltration Centrifugal Tube (Akayunguruzo ka Centrifugal)Ibicuruzwa bikurikirana
Igizwe n'umuyoboro w'imbere (hamwe na membrane) + umuyoboro wo hanze wa centrifuge, ushobora kweza vuba kandi neza kandi ukagera kuri 15ml by'ibinyabuzima. Igishushanyo cyihariye cya vertical hamwe nubuso bunini bwo kuyungurura birashobora gutanga ubushobozi bwihuse bwo gutunganya hamwe nigipimo cyinshi cyo kugarura ibintu (mubisanzwe ≥90% byoroheje igisubizo cyumwimerere), mugihe gikomeza gushungura byoroheje hamwe nibidukikije kugirango bikomeze ibikorwa bya poroteyine no Guhinduka. Igishushanyo cyihariye gishobora guhindagurika. igisubizo kandi kigabanya cyane ikosa rya filteri membrane iterwa na centrifugation ikurikira. Muguhindura uburyo bwa filteri yumubiri ihagarara mugikoresho cyo kuyungurura, irashobora kubuza gushungura centrifugal kumisha icyitegererezo cyangwa gutera igihombo cyicyitegererezo kubera kuzenguruka cyane. . Ibice bitandukanye bya ultrafiltration ya molekuline yuburemere (MWCO) ya santrifugali ikoresha filtre ikoresha ubuziranenge bwinshi, adsorption nkeya PES na RCE membrane, ifite proteine nkeya cyane hamwe na aside nucleic aside ihuza.
BM Ubuzima Ubumenyi, ibyumba bisukuye mubyiciro byose byaUltrafiltration Centrifugal Tube (Akayunguruzo ka Centrifugal), imikorere yumurongo, imikorere ya ERP yuzuye, ibicuruzwa birenze urugero, nta DNase / RNase, nta PCR ibuza, nta soko yubushyuhe. Ubuzima bwa BM Ubumenyi, ingano zose zaUltrafiltration Centrifugal Tube (Akayunguruzo ka Centrifugal)Ibicuruzwa byuruhererekane birateguwe, urukurikirane rwibicuruzwa ruhagaze neza hamwe nibitandukaniro rito hagati yicyiciro kandi murwego rwo hejuru. Bashobora gusimburwa n’ibicuruzwa byo mu gihugu no koherezwa mu Buyapani, Koreya yepfo, Uburayi, na Amerika. Byakoreshejwe cyane mubisanzwe icyitegererezo cyo gutandukanya no gushungura.
Gusaba
1 UltrafiltrationGusimbuza Buffer, kweza, kwibanda, no gusibanganya antigene, antibodies, enzymes, nizindi poroteyine, hamwe nubushakashatsi bwibinyabuzima nka acide nucleic acide na mikorobe, kuvanaho ibice bya chromatografique, gutandukanya molekile yibinyabuzima n'umuco wa tissue cyangwa ibisubizo bya lysis selile, kuvanaho ya primers, umuhuza, cyangwa ibimenyetso bya molekuline bivuye kuvanga reaction, kwibanda kuri virusi cyangwa gutandukana, no gutandukanya byimazeyo ivangwa rya molekile ya biologiya.
2 Microfiltrationitandukanya acide nucleic na proteyine muri geles, ingero zirayungurura kandi zirasobanurwa, kandi ibice bitunganijwe cyangwa amasaro byegeranijwe bikaraba.
Ikiranga ibicuruzwa
★Ikigereranyo cyo hejuru cyane, gishobora kugera ku nshuro 80-100 igipimo cyo kwibanda ;
★Icyitegererezo cyo kwibandaho cyihuta, kandi igihe cyo kwibandaho ni iminota 10-60. Ifite kandi anti-yumye igishushanyo mbonera cyo kwirinda kwangirika kwicyitegererezo kubera centrifugation ikabije ;
Rate Icyitegererezo cyo gukira ni kinini kandi gishobora kugera ku gipimo cyo gukira kirenga 90% ;
Ad adsorption nkeya, PES / RC membrane hamwe nurukuta rwimbere rwimbere rufite poroteyine nkeya cyane hamwe na biomolecule ihuza igipimo ;
4ml / 15ml 、 15ml / 50ml
Ibisobanuro byuzuye, ultrafiltration centrifuge tubes ifite umubumbe wa 4ml / 15ml na 15ml / 50ml, hamwe nubunini butandukanye bwa pore, iboneka muri 3KD, 5KD, 10KD, 30KD, 50KD na 100KD kugirango wuzuze ibisabwa bitandukanye byo kugabanya ibiro bya molekile ;
Operation Ibikorwa byoroshye, gutandukana kwose hamwe no kuyungurura birashobora kurangizwa hamwe naCentrifuge, byoroshye gukora ;
Ibicuruzwa bifite isuku, amahuza yose akorerwa mucyumba gisukuye, imikorere yumurongo winteko, imiyoborere yuzuye ya ERP, ibicuruzwa ni ultra-yera, nta DNase / RNase, nta PCR ibuza, nta soko yubushyuhe;
Definition Ibisobanuro bihanitse, nta background na auto-fluorescence, kandi ntabwo bigira ingaruka kubisubizo;
Performance Imikorere yizewe, gufunga neza, kandi ntigire icyo ikora hamwe nubushakashatsi bwakozwe, ibisubizo byubushakashatsi byizewe, kandi birashobora kwihanganira ubushyuhe bwo hejuru bwa 100 ℃!
★ Ifite intera nini ya porogaramu. Kuri Porogaramu zitandukanye, ubwoko bubiri bwaUltrafiltration Centrifugal Tube (Akayunguruzo ka Centrifugal)ibicuruzwa bikurikirana, 4ml / 15ml na 15ml / 50ml byamenyekanye kugirango bikemure abakiriya batandukanye, bemera kwihitiramo no guteza imbere imikorere!
Tegeka amakuru
Injangwe. Oya | Izina | Ibisobanuro | Ibisobanuro | Pcs / pk |
UCT015RCE004003 | Ultrafiltration Centrifugal Tube | 4 / 15ml , 3kD | , RCE Membrane | 15 pc / igikapu |
UCT015RCE004010 | Ultrafiltration Centrifugal Tube | 4 / 15ml , 10kD | RCE Membrane | 15 pc / igikapu |
UCT015RCE004030 | Ultrafiltration Centrifugal Tube | 4 / 15ml , 30kD | RCE Membrane | 15 pc / igikapu |
UCT015RCE004100 | Ultrafiltration Centrifugal Tube | 4 / 15ml , 100kD | RCE Membrane | 15 pc / igikapu |
UCT050RCE015003 | Ultrafiltration Centrifugal Tube | 15 / 50ml , 3kD | RCE Membrane | 24 pc / igikapu |
UCT050RCE015010 | Ultrafiltration Centrifugal Tube | 15 / 50ml , 10kD | RCE Membrane | 24 pc / igikapu |
UCT050RCE015030 | Ultrafiltration Centrifugal Tube | 15 / 50ml , 30kD | RCE Membrane | 24 pc / igikapu |
UCT050RCE015100 | Ultrafiltration Centrifugal Tube | 15 / 50ml , 100kD | RCE Membrane | 24 pc / igikapu |
UCT050PES015005 | Ultrafiltration Centrifugal Tube | 15 / 50ml , 5kD | PES Membrane | 12 pc / igikapu |
UCT050PES015010 | Ultrafiltration Centrifugal Tube | 15 / 50ml , 10kD | PES Membrane | 12 pc / igikapu |
UCT050PES015030 | Ultrafiltration Centrifugal Tube | 15 / 50ml , 30kD | PES Membrane | 12 pc / igikapu |
UCT050PES015050 | Ultrafiltration Centrifugal Tube | 15 / 50ml , 50kD | PES Membrane | 12 pc / igikapu |
UCT050PES015100 | Ultrafiltration Centrifugal Tube | 15 / 50ml , 100kD | PES Membrane | 12 pc / igikapu |
CF050PES015C010 | Akayunguruzo | 15 / 50ml , 0.1um | 0.1um , PES Membrane | 12 pc / igikapu |
CF050PES015C020 | Akayunguruzo | 15 / 50ml , 0.2um | 0.2um , PES Membrane | 12 pc / igikapu |
CF050PES015C045 | Akayunguruzo | 15 / 50ml , 0.45um | 0.45um , PES Membrane | 12 pc / igikapu |
UCT ** | Ultrafiltration Centrifugal Tube | Guhitamo | Guhitamo | ** pcs / igikapu |
CF ** | Akayunguruzo | Guhitamo | Guhitamo | ** pcs / igikapu |
Ubuzima bwa BM, Ultrafiltration Centrifugal Tube (Centrifugal Filter) ibipimo byibicuruzwa hamwe namakuru yubushakashatsi
Ultrafiltration Centrifugal Tube (Centrifugal Filter) ikurikirana ryibicuruzwa hamwe namakuru yubushakashatsi
Ultrafiltration Centrifugal yintangarugero:
5 KD 0.25mg / ml Cyt C ;
10 KD1mg / ml OVA ;
30/50 KD 1mg / ml BSA ;
100 KD 1mg / ml IgG。
Imiterere yubushakashatsi: rotor ya horizontal, imbaraga za centrifugal 4000 × g, 8 ℃, gutangira ingano 15ml.
Ultrafiltration Centrifugal yintangarugero:
5 KD 0.25mg / ml Cyt C ;
10 KD 1mg / ml OVA ;
30/50 KD 1mg / ml BSA ;
100 KD 1mg / ml IgG.
Imiterere yubushakashatsi: inguni ihamye, imbaraga za centrifugal 5000 × g, 8 ℃, gutangira ubunini bwa 12ml.
Igipimo gisanzwe cyo kwangwa kubipimo byuburemere bwa poroteyine (4000g x 15ml ingano yo gutangira rotor cyangwa 5000g x 12ml yo gutangira ingano ya rotor ihamye)
ibipimo by'uburemere bwa molekuline / kwibanda | uburemere bwa molekile | NMWL Ultrafiltration tube | igipimo cyo kwangwa% Shiraho indobo | Igipimo cyo kwangwa% inguni ihamye | igihe cya centrifugation |
B-12 vitamine | 1350 | 3K | < 25 | < 25 | 60 |
(0.25mg / ml) Cytochrome C. | 12400 | > 95 | > 95 | 60 | |
(1mg / ml) α-Chymotrypsinogen | 25000 | > 95 | > 95 | 60 | |
B-12 (0.2mg / ml) vitamine | 1350 | 10K | < 5 | < 5 | 30 |
0,25mg / ml) Cytochrome C. | 12400 | > 95 | > 95 | 30 | |
(1mg / ml) α-Chymotrypsinogen | 25000 | > 95 | > 95 | 30 | |
vitamine B-12 (0.2mg / ml) | 1350 | 30K | < 5 | < 5 | 20 |
Cytochrome C 0.25mg / ml) | 12400 | < 10 | < 10 | 20 | |
Ovalbumin (1mg / ml) | 45000 | > 95 | > 95 | 20 | |
BSA (1mg / ml) | 67000 | > 95 | > 95 | 20 | |
Cytochrome C (0,25mg / ml) | 12400 | 50K | < 5 | < 5 | 10 |
Ovalbumin (1mg / ml) | 45000 | 65 | 55 | 10 | |
BSA (1mg / ml) | 67000 | > 90 | > 90 | 10 | |
Ovalbumin (1mg / ml | 45000 | 100K | < 25 | < 15 | 20 |
IgG (1mg / ml) | 156000 | > 90 | > 90 | 20 | |
Thyroglobuline (0.5mg / ml) | 677000 | > 90 | > 90 | 20 |
Igipimo cyo gukira
Ubushobozi bwo kugumana 15ml ultrafiltration centrifuge tube membrane isobanurwa no kugabanya uburemere bwa molekile (MWCO).
Ibipimo bya molekuline byuzuye hafi ya MWCO birashobora kugumana igice gusa. Kugirango ubone ibyiza byo kugumana,
nyamuneka koresha MWCO muyunguruzi ya membrane ifite uburemere bwa 1/3 cyangwa munsi ya solute.
Kugereranya igipimo cyo kugarura ibicuruzwa
Kugereranya ibipimo byo kugarura poroteyine nubunini bwapfuye bwibirango bitandukanye bya ultrafiltration tubes
10KD Kugereranya ibipimo byo kugarura poroteyine kandi byapfuye ingano yibirango bitandukanye bya ultrafiltration tubes | |||
Ikirango | (% Rate Igipimo cyo gukira | (Μl volume Ingano yapfuye | |
BM | 1㎎ / ml Intungamubiri za poroteyine | 90 | 87 concent Intungamubiri za poroteyine1㎎ / ml) |
0.1㎎ / ml Intungamubiri za poroteyine | 83 | ||
Ikirango M. | 1㎎ / ml Intungamubiri za poroteyine | 93 | 47 concent Intungamubiri za poroteyine1㎎ / ml) |
0.1㎎ / ml Intungamubiri za poroteyine | 88 | ||
Ikirango S. | 1㎎ / ml Intungamubiri za poroteyine | 91 | 70 concent Intungamubiri za poroteyine1㎎ / ml) |
0.1㎎ / ml Intungamubiri za poroteyine | 85 | ||
Ikirango K. | 1㎎ / ml Intungamubiri za poroteyine | 89 | 186 concent Kwibanda kuri poroteyine1㎎ / ml) |
0.1㎎ / ml Intungamubiri za poroteyine | 77 | ||
Ikirango J. | 1㎎ / ml Intungamubiri za poroteyine | 83 | |
0.1㎎ / ml Intungamubiri za poroteyine | 50 | ||
Imiterere yubushakashatsi: poroteyine ya OVA (uburemere bwa molekile 45KD), rotor ya horizontal 4000g | |||
Ibyatoranijwe guhitamo ibisobanuro bya ultrafiltration tubes ifite uburemere butandukanye bwa molekile
ingano yo gutangira | < 0.5ml | < 2ml | < 4ml | < 15ml | |
Poroteyine | |||||
(MW) uburemere bwa molekile | MWCO (Da) kugabanya uburemere bwa molekile | ||||
6K < MW < 20K | 3000 | 3000 | 3000 | 3000 | |
20K < MW < 60K | 10000 | 10000 | 10000 | 10000 | |
60K < MW < 100K | 30000 | 30000 | 30000 | 30000 | |
100K < MW < 200K | 50000 | 50000 | 50000 | 50000 | |
200K < MW | 100000 | 100000 | 100000 | 100000 | |
Acide nucleic-imwe imwe hamwe na acide nucleic acide | |||||
uburebure | MWCO (Da) kugabanya uburemere bwa molekile | ||||
137-1159bp | 3000 | 3000 | 3000 | 3000 | |
nanoparticles | |||||
IA DIA size ingano yubunini | MWCO (Da) kugabanya uburemere bwa molekile | ||||
1.5 < DIA < 3nm | 3000 | 3000 | 3000 | 3000 | |
3 < DIA < 5nm | 10000 | 10000 | 10000 | 10000 | |
5 < DIA < 7nm | 30000 | 30000 | 30000 | 30000 | |
7 < DIA < 10nm | 50000 | 50000 | 50000 | 50000 | |
10 nm < DIA | 100000 | 100000 | 100000 | 100000 |
Igihe cyo kohereza: Gashyantare-18-2024