Uburyo bwakweza poroteyine:

Uburyo bwo kweza poroteyine, gutandukanya no kweza poroteyine, poroteyine irekurwa mu ngirabuzimafatizo cyangwa ingirangingo z'umwimerere mu buryo bwashonze kandi ikaguma mu mwimerere karemano nta gutakaza ibikorwa by’ibinyabuzima. Kubera iyo mpamvu, ibikoresho bigomba kuvanaho inyamanswa na adipose, ibikoresho byimbuto bigomba no gukuraho kwanduza ikoti ryimbuto mubintu nka tannine, kandi imbuto yamavuta igomba guhitamo gukoresha ibishishwa bito bitetse.

1. density gradient centrifugation Ubwiza nubucucike bwa poroteyine hagati bizagira ingaruka zikomeye kubice. Gukemura umuvuduko birihuta; gel muyunguruzi ni ubwoko bwinkingi chromatografiya.

2. igisubizo cyumunyu gikoreshwa numuti wumunyu Ubwiyongere bwiyongera cyangwa bugabanya gukomera kwa poroteyine.

3. Amafaranga yishyurwa aratandukanijwe kandi asesengurwa muburyo butandukanye, ibyingenzi birimo electrophoreis na ion yo guhana chromatografiya.

4. Uburyo bwakweza poroteyineGuhitamo adsorption no gutandukanya poroteyine zikoresha imbaraga za adsorption.

5. Affinity chromatography ishingiye ku gutandukanya ligande. Ibi biranga ibinyabuzima byemerera molekile za poroteyine guhuza byumwihariko aho guhurira nindi molekile yitwa ligand.

6. Ubu buryo buri hejuru kurenza imyunyu ngugu, ariko poroteyine yerekana byinshi Iyo byoroshye, bigomba gukorwa mubushyuhe buke.

Uburyo bwo kwezakweza poroteyine:

Nigute ushobora kweza poroteyine, proteyine ni molekile nini, hamwe nubunini butandukanye bwa alubumu, hariho uburyo bworoshye bwo gutandukanya poroteyine nibikoresho bito bya molekile, ndetse no gutandukanya poroteyine. Uburyo nyamukuru bwo gutandukanya poroteyine zingana na molekuline zitandukanye ni dialyse, ultrafiltration, gel filtration, hamwe no gutandukanya centrifugal. Dialysis na ultrafiltration bikoreshwa muburyo bwo gutandukanya poroteyine. Uruvangitirane rwa dialyse rugomba gutandukana rushyirwa mumufuka wa dialyse wakozwe na semipermeable membrane, hanyuma ukinjizwa mumuti wa dialyse kugirango utandukane. Ultrafiltration ninzira yo gutambutsa amazi nizindi molekile ntoya ukoresheje imbaraga za centrifugal cyangwa igitutu muri kimwe cya kabiri cyinjira no gufata proteine ​​mugice cya kabiri cyinjira. Ubu buryo bubiri bushobora gutandukanywa na macromolecules ishingiye kuri poroteyine na molekile ntoya ishingiye ku munyu. Bakunze kuba umunyu, ugakoreshwa muburyo bwo gusesa umunyu, cyangwa urashobora gutangizwa kugirango ukoreshe ubu buryo bubiri nyuma yo gushiramo umunyu ukoresheje ibisubizo byumunyu ngugu.

. ibikorwa by'amazi biziyongera. Kugabanuka, ibyo na byo bikaba bishobora gutuma poroteyine zimwe na zimwe zitanga amafaranga binyuze mu miterere ya molekuline igenda itabangikanya buhoro buhoro, kandi na hydration membrane igenda isenywa buhoro buhoro, amaherezo bikaba bishobora gutera poroteyine na molekile bifitanye isano no gukura hamwe no guturuka ku bisubizo bitandukanye.

2. icya kabiri, dielectric ihoraho yumuti ukomoka kumazi kuruta amazi irashobora kugabanuka Mumashanyarazi.

3. Intungamubiri za poroteyine: poroteyine nk'imvura igwa ikora ku bwoko bumwe cyangwa bumwe mu mvura ya poroteyine, ibisanzwe ni alkaline, poroteyine, inyigisho, hamwe n'ibyuma biremereye.

4. Imvura ya polyethylene glycol: Nigute ushobora kweza amazi adashonga amazi atari ionic polymerskweza poroteyine, nka polyethylene glycol na sodium dextran sulfate kugirango igabanye poroteyine.