Icupa ry'icyitegererezo ni ikintu cyo gusesengura ibikoresho byo gusesengurwa, kandi isuku yacyo igira ingaruka ku bisubizo by'isesengura. Iyi ngingo ivuga muri make uburyo butandukanye bwo gusukura icupa rya chromatografique, kandi rigamije gutanga ibisobanuro bifatika kuri buri wese. Ubu buryo bwagenzuwe ninshuti nabababanjirije. Zifite ingaruka nziza zo gukaraba kubisigazwa byamavuta hamwe nibisigara bya reagent muriicupa rya chromatografi. Isuku yujuje ibisabwa, intambwe zo gukora isuku ziroroshye, kandi igihe cyogusukura kiragabanuka, kandi inzira yisuku irangiza ibidukikije.

Nyamuneka hitamo ukurikije uko laboratoire yawe imeze!

Kugeza ubu, hamwe n’inyungu ziyongera ku bwiza bw’ibiribwa n’umutekano biturutse mu nzego zose, ikoranabuhanga ryisesengura rya chromatografi rirakoreshwa cyane mu gupima ubuziranenge bw’ibiribwa no gupima umutekano, cyane cyane mu rwego rwo gupima ibicuruzwa by’ubuhinzi, ikoranabuhanga rya chromatografique ryakoreshejwe cyane. Mu gihugu cyanjye, umubare munini wibikomoka ku buhinzi (ibindi bicuruzwa bivura imiti, acide organic, nibindi) bigomba gupimwa na chromatografiya y’amazi na gazi ya chromatografiya buri mwaka. Bitewe numubare munini wicyitegererezo, hariho umubare munini wamacupa yintangarugero agomba gusukurwa mugihe cyo gutahura, bidatakaza umwanya gusa kandi bikagabanya imikorere yakazi, ariko kandi rimwe na rimwe bitera gutandukana mubisubizo byubushakashatsi bitewe nisuku ya amacupa yicyitegererezo.

Uwitekaicupa rya chromatografiqueikozwe cyane mubirahure, gake cyane plastike. Amacupa yintangarugero yajugunywe ahenze, arasesagura, kandi atera umwanda ukabije wibidukikije. Laboratoire nyinshi zisukura amacupa yintangarugero hanyuma ukongera kuyakoresha. Kugeza ubu, uburyo bukunze gukoreshwa muri laboratoire kugira ngo usukure icupa ry'icyitegererezo ahanini ni ukongeramo ifu yo kumesa, detergent, solvent organic, hamwe na lisansi ishingiye kuri acide, hanyuma ugasukuza hamwe nigituba gito cyabigenewe. Ubu buryo busanzwe bwo gushakisha bufite inenge nyinshi. Ikoresha ibintu byinshi byogejwe n'amazi, bifata igihe kinini cyo gukaraba, kandi ikunda gusiga inguni zapfuye. Niba ari icupa ryicyitegererezo cya plastiki, biroroshye gusiga ibimenyetso bya brush kurukuta rwamacupa yimbere, bifata abakozi benshi. Kubirahuri byanduye cyane ibisigazwa bya lipide na proteyine, umuti wa alkaline lysis ukoreshwa mugusukura, kandi ibisubizo byiza biragerwaho.

Iyo usesenguye ingero, gusukura icupa ryatewe ni ngombwa cyane. Ukurikije uburyo bwo koza ibirahuri, uburyo bwo gukora isuku bwatoranijwe ukurikije urugero rw’umwanda, kandi nta buryo buhamye. Incamake yuburyo:

1. Suka igisubizo cyikizamini mu icupa ryumye

2. Shira byose muri alcool 95%, kwoza kabiri hamwe na ultrasonic hanyuma ubisuke, kuko inzoga zinjira byoroshye mumashanyarazi ya 1.5mL kandi birashobora kutabangikanywa numuti mwinshi kama kugirango ugere kubikorwa byogusukura.

3. Suka mumazi meza, hanyuma ukarabe ultrasonique kabiri.

4. Suka amavuta yo kwisiga mumacupa yumye hanyuma uteke kuri dogere selisiyusi 110 mumasaha 1 ~ 2. Ntuzigere uteka ku bushyuhe bwinshi.

5. Nkonje kandi uzigame.