Isesengura rya Blot muri Biopharmaceutical, Medical and other Field
Gahunda ya 14 y’imyaka itanu "Gahunda y’iterambere ry’ibinyabuzima ivuga ko ubukungu bugomba guterwa n’iterambere n’iterambere ry’ubumenyi bw’ubuzima n’ibinyabuzima, bishingiye ku kurinda, guteza imbere no gukoresha umutungo w’ibinyabuzima, kandi bishingiye ku guhuza kwinshi kandi kwimbitse ubuvuzi, ubuzima, ubuhinzi, amashyamba, n'ingufu. , kurengera ibidukikije, ibikoresho n’inganda zindi; biragaragara ko iterambere rya bio-ubukungu nicyerekezo cyingenzi cyo kubahiriza uburyo bwihuse bwihindagurika ryibinyabuzima ku isi no kugera ku rwego rwo hejuru siyanse na tekinoloji yo kwigira. Nigipimo cyingenzi cyo guhinga no kwagura bio-nganda no guteza imbere ubukungu bufite ireme. Guhura n’iterambere ryihuse ry’ubuzima n’ibikenewe mu buzima no guhaza ibyifuzo by’abaturage kugira ngo babeho neza ni ingwate ikomeye yo gushimangira gukumira no gukumira ingaruka z’umutekano w’umutekano w’igihugu no guteza imbere ivugurura ry’imiyoborere y’igihugu n’ubushobozi bw’imiyoborere.
Mu gusubiza umuhamagaro w’igihugu, BM yiyemeje gutsinda ikoranabuhanga ryo mu rwego rwo hejuru kandi rikanamenya buhoro buhoro gusimbuza ibicuruzwa biva mu mahanga bifite agaciro gakomeye mu bumenyi bw’ubuzima. Muri Gicurasi 2023, umusaruro mwinshi wa immunochromatography NC membrane wagerwaho neza kandi ukoreshwa muburyo butandukanye bwihuse. Kugeza ubu, filime ya NC yakoreshejwe mu gihugu mu gusuzuma indwara ya vitro, umutekano w’ibiribwa, kwipimisha byihuse n’ibindi bice, kandi imaze kugera ku bicuruzwa byoherezwa mu mahanga kandi irushanwa n’ibihangange mpuzamahanga ku isoko! Nyuma yo kurangiza ikiganiro cyamasoko ya firime ya NC, nyuma y amezi menshi yubushakashatsi bwa tekiniki nitsinda ryacu rya tekiniki, kugirango dusubize byihutirwa abakoresha murwego rwubumenyi bwubuzima bwisi yose kugirango bagabanye igiciro cyibintu byingenzi bifite agaciro gakomeye, twatangije neza blotting membrane. , ikwiranye na biofarmaceuticals, ubuvuzi nizindi nzego. Isesengura ryiburengerazuba (Blotting yuburengerazuba, WB)
Iriburiro ryibiranga BM Blotting Membranes ,: Ingano ya pore nubwoko bwa poroteyine ikoreshwa 0.1μm ikwiranye na poroteyine zifite uburemere bwa molekile munsi ya 7kDa 0.22μm ikwiranye na poroteyine zifite uburemere bwa molekile munsi ya 20kDa 0.45μm ikwiranye na poroteyine zifite uburemere burenze 20kDa Main Amahame ya poroteyine ahuza Amashanyarazi ahamye hamwe na hydrophobicity Uburyo bukoreshwa bwo kwimura hamwe nuburyo bwo gutahura Chemiluminescence Fluorescence detection radiolabel probe itaziguye irangi rya Enzyme ifitanye isano na antibody Inyungu ：
1.Kuri inyuma, ibyiyumvo byinshi
2.Ntabwo ukeneye inzoga reagent mbere yo guhanagura
3.Ubuso bwihariye bwimiterere nibintu bitera ibimenyetso byiza-byerekana urusaku Ibikoresho bikomoka kuri fibre naturel, byangiza ibidukikije, kandi birashobora gutuma poroteyine ihambiriye ikora igihe kirekire.
Iriburiro ryikoranabuhanga rya WB isesengura rya tekinoroji ya WB ni ikoranabuhanga rikoreshwa cyane mubijyanye na biyolojiya y’ibinyabuzima, ibinyabuzima, ibinyabuzima, ubudahangarwa n’izindi nzego. Iri koranabuhanga rikoresha uburyo bwihariye bwo guhuza antibodi na poroteyine zihariye ziri mu ngirabuzimafatizo cyangwa ingirabuzimafatizo kugira ngo ugere kuri poroteyine no gusesengura imvugo ukurikije umwanya n'uburemere bw'ibara ry'amabara, ni ukuvuga isesengura ryujuje ubuziranenge na kimwe cya kabiri. Yatanzwe bwa mbere na Harry Towbin wo mu kigo cya Friedrich Miescher mu Busuwisi mu 1979. Ubu hashize imyaka irenga 40 kandi kikaba uburyo bwa kera kandi bukora ubushakashatsi bwa poroteyine.
Igihe cyo kohereza: Gashyantare-02-2024
