Ni-NTA Preloaded Columns&Plates

①Mwatsatanetsatane mankhwala magawo

Gulu lazogulitsa: Mzere/mbale ya Ni-NTA yokonzekera zitsanzo za ma antibody a protein peptide ndi kuyeretsa mapuloteni

zakuthupi: PP + Agarose gel

Voliyumu: 1ml, 3ml, 5ml, 6ml, 12ml ndi 2ml 96-hole desalination ndi mbale zoyeretsera

Ntchito: olimba gawo m'zigawo za mapuloteni polypeptide antibody, kusefera, adsorption, kulekana, m'zigawo, kuyeretsa ndi ndende ya chandamale zitsanzo

Zofotokozera: 0.5ml/1ml, 1ml/3ml, 1.5ml/3ml, 1.5ml/5ml, 2.5ml/5ml, 2ml/6ml, 3ml/6ml, 4ml/12ml, 6ml/12ml,×96, 0.8ml×96

Kupaka: 25 zidutswa / 1ml, 20 zidutswa / 3ml, 30 zidutswa / 6ml, 20 zidutswa / 12ml, 5 zidutswa / bokosi sing'anga chromatography ndime, 1 chidutswa / paketi ya 96-hole desalination ndi mbale kuyeretsedwa

Zakuyikapo: thumba la aluminium zojambulazo kapena thumba la opaque aluminium zojambulazo (ngati mukufuna)

Bokosi loyika: bokosi lamakalata osalowerera kapena bokosi lamtundu wa BML (ngati mukufuna)

makonda a Logo: inde

kupereka mode: OEM/ODM

②Mafotokozedwe Akatundu

BM life science Ni-NTA column idayeretsedwa ndi protein affinity chromatography ndi agarose as crosslinking medium.Mfundo yoyeretsera ndikuyeretsa puloteni yomwe mukufuna ndi tag pogwiritsa ntchito chinthu chomwe chimamangiriza pakati pa ma nickel ions chelated pa NTA ndi tag.Njira iyi ndi yosavuta kugwiritsa ntchito. , kuyeretsedwa kwakukulu, kosavuta kuyeretsa kwakukulu kwa mapuloteni ofunikira.

Nickel chloride mu gawo la Ni amatha kumangika ku mapuloteni ndi chizindikiro chake (histone). Pambuyo kumanga, zachilengedwe chandamale mapuloteni akhoza anapezerapo ndi imidazole elution.Packing magawo a Ni ndime mu zana mailosi moyo sayansi.

Gawo laling'ono: 4% kapena 6% agarose olumikizidwa;

Avereji tinthu kukula: 90um (45-165um); yosungirako njira: 20% Mowa njira;

PH kukhazikika: 2-12;

Ma denaturants osamva: 6M guanidine hydrochloride ndi 8M urea;

Kupirira kupanikizika: <0.3mpa, 0.1mpa ikulimbikitsidwa;

Adsorption:> 20mg(27kDa) mapuloteni / ml;

Zosungirako: 2-8℃.

BM Life Science Ni ndime yodzaza ndi zinthu 5, kuphatikiza 1, 3, 5, 6 ndi 12ml. 1ml ndi 5ml ndi chisanadze odzaza mizati ya sing'anga kuthamanga chromatography, amene angagwiritse ntchito mokwanira ubwino wa sing'anga kuthamanga madzi gawo kuyeretsedwa dongosolo mofulumira ndi kothandiza desalination ndi kuyeretsa kwachilengedwenso macromolecules.

③Zogulitsa:

★zosiyanasiyana specifications: 1/3/6/12ml syringe, 1/5ml kwa sing'anga chromatography column;

★Kuthamanga kwapang'onopang'ono: kupanikizika kwapakati pa chromatography yokhazikitsidwa kale ndime yololera mpaka 0.6mpa (6 bar, 87 psi);

★yosavuta kugwiritsa ntchito: mawonekedwe a ruer, angagwiritsidwe ntchito mndandanda kuti awonjezere kutsitsa kwachitsanzo, amathanso kugwirizanitsidwa ndi syringe ndi pampu ya peristaltic, akhoza kulumikizidwanso mwachindunji KTA, Agilent, Shimadzu, Waters ndi machitidwe ena oyeretsera gawo lamadzi;

★amagwiritsidwa ntchito kwambiri: oyeretsedwa nucleic acid, ma antibodies, mapuloteni olembedwa, kutulutsa mchere.

Order Information

Mphaka Nambala yofotokozera mtundu (ml) kulongedza

PNI001-1 wofiira / wobiriwira NI sing'anga kuthamanga chromatography chisanadze anaika ndime 1 5 / bokosi

PNI001-2 mandala chubu / wofiira chivundikiro NI syringe mtundu preloaded ndime 1 25 / bokosi

PNI003-1 mandala chubu / wofiira chivundikiro NI syringe mtundu chisanadze odzaza ndime 3 20 / bokosi

PNI005-1 wofiira / wobiriwira NI sing'anga chromatography anaikapo ndime 5 5 / bokosi

PNI006-1 mandala chubu / wofiira kapu NI syringe mtundu chisanadze odzaza ndime 6 30 / bokosi

PNI012-1 mandala chubu / wofiira chivundikiro NI syringe mtundu chisanadze odzaza ndime 12 20 / bokosi

BM Life Science, mndandanda wonse wa machubu a SPE amapangidwa ndi jekeseni wamankhwala a polypropylene; mbale ya sieve imapakidwa ndi polyethylene.sorbents yaposachedwa kwambiri ya polyethylene.sorbents yogulidwa padziko lonse lapansi, ndikuwunika kovomerezeka, mtundu ndi wodalirika; 10,000 kupanga kalasi koyeretsa, njira zopangira zokhazikika, kasamalidwe ka ERP, kutsatiridwa kwamtundu wazinthu; mitundu yosiyanasiyana yazinthu kuti ikwaniritse zosowa zamakasitomala; Zogulitsa zathu zonse zimasinthidwa makonda kuti makasitomala azisangalala ndi ntchito zapamwamba zoyimitsa kamodzi.

Zina mwazinthu zamtundu wa BM Life Science SPE

Ubwino waukadaulo:

★Ma sorbents ena a SPE ofufuza ndi chitukuko ndi kupanga ukadaulo wokhala ndi ufulu wodziyimira pawokha waluntha, kutsitsa kwa SPE (kugawa ufa, kutsitsa, kulongedza) kumangochitika zokha.

★Kudalira ubwino wapadera wa ndende nkhungu CNC jekeseni akamaumba makampani mu ngale mtsinje delta, kuphatikiza ndi bwino magwiritsidwe ntchito chuma kwawirikiza kawiri mphamvu yopanga mapaipi ndi SPE ndime, ndi theka mtengo wa nkhungu akamaumba ndi jekeseni akamaumba, ndi bwino kwambiri khalidwe la mankhwala

★Kampaniyo ili ndi luso lapadera laukadaulo laukadaulo wogawa ufa wabwino, kugawa ufa kotheratu munjira yodziwikiratu, yosasunthika, yosasunthika kuti iwonetsetse kuti gulu lazinthu likhale lokhazikika.

★Mazana a mamiliyoni a R&D ndi mphamvu yopangira mbale za sieve ndi zosefera zachepetsa mtengo wopanga SPE mpaka wotsika kwambiri.

★Sieve mbale ya SPE ndiyodziyimira pawokha, m'mimba mwake, makulidwe ake, kukula kwa kabowo kumatha kusankhidwa ndikuphatikizidwa mwakufuna kwake.

★kampaniyo imaona kufunikira kwakukulu kwa luso laukadaulo ndi kuwongolera kosalekeza, makamaka Tip SPE, sieves free panel inlaying SPE, 96-384-hole plate SPE yokhala ndi ufulu wodziyimira pawokha waluso, womwe umadzaza kusiyana ku China ndikufikira pamlingo wapadziko lonse lapansi, kuwonetsa Ubwino wapadera wa B&M Life Science mu gawo la SPE.

Ubwino wazinthu:

★Yosavuta kugwiritsa ntchito, pansi pa mphamvu yokoka yachilengedwe imatha kukwaniritsa kuchuluka kwa liwiro komanso kubweza bwino

★Itha kupulumutsa mtengo wa zida ndi zogwiritsidwa ntchito kwambiri popanda SPE ndi zida zovumbula

★sorbent yoyera popanda kusokonezedwa ndi maziko opanda kanthu

★mkulu wobwezeretsanso mlingo wa 10 ~ 100ppm unali wabwino kwambiri wa 95% ~ 105%

★Ndi mphamvu yayikulu yotsatsa, ndiyopambana kuposa mitundu ina ya SPE ku China

★Khalidwe lazinthu zokhazikika, kupangika bwino, kupatuka kwachibale (RSD) <5%

★Osawopa ndime yowuma. Kuwuma ndi kunyowa kumafanana mkati mwazolakwika, kusiyana kwachibale (RSD) <0.05%

★Zogulitsa zathu zili pamlingo womwewo monga Waters / Agilent/ Supelco ndi makampani ena

★Kutsika kwamitengo yazinthu zathu ndi imodzi mwazabwino kwambiri padziko lonse lapansi

Ntchito:

★Amagwiritsidwa ntchito kwambiri m'nthaka;Mafuta;madzimadzi am'thupi (plasma/mkodzo, etc.);Chakudya ndi zina zotero.

Kudzipereka kwabwino:

★Onetsetsani kuti chinthu chilichonse ndi choyenera, tsatirani mfundo zoyendetsera bwino, tsatirani kuwunika kwa batch

★Onetsetsani kuti mankhwala aliwonse alibe kusokoneza opanda kanthu, mlingo wa kuchira bwino kuposa malamulo a dziko, kufika mlingo wapamwamba wa mankhwala ofanana.

Malonjezo a magwiridwe antchito:

★Perekani chithandizo chaukadaulo kwaulere

Gawo la SPE

Polima matrix mndandanda SPE

Ndi ozungulira polima monga adsorbent, sorbent tinthu kukula ndi yunifolomu, SPE ndime otaya mlingo ndi khola, amene ndi tingachipeze polima ndime mu labotale, mndandanda wonse wa mankhwala akhala ankagwiritsa ntchito kuyesa chakudya. Zogulitsa ndizofanana ndi Waters' HLBS, MAX's ndi MCX's.

Silica gel matrix mndandanda wa SPE

Classic silica gel matrix SPE column, amorphous / spherical adsorbent, yokhala ndi mtengo wokwera komanso wodziwa bwino wogwiritsa ntchito ndi kasitomala trust.Zogulitsa zimagwiritsidwa ntchito kwambiri pakuwunika mankhwala.

Langizo m'zigawo / kuyeretsa / ndende ya SPE

ndi chida choponyera nthungo chomwe chimagwiritsidwa ntchito popangira / kuyeretsa kukulitsa zitsanzo zachilengedwe kapena Tip of SPE, cholinga chopangira mikondo pamwamba kuwonjezera C4 / C18 / silicon powder/magnetic beads/ProtingA (G) agarose gel sorbent, yogwiritsidwa ntchito primer/genomic DNA plasmid PCR product/peptide protein/antibody to target product/sefa/extraction/desalting/purification/kulemeretsa.

96/384-bowo mbale mbale SPE

The 96/384-well mbale mndandanda SPE opangidwa makamaka mkulu-throughput chitsanzo pretreatment.Complete chitsanzo chisanadze ndondomeko ndondomeko ndi kampani orifice fyuluta kapena ntchito basi.

Special kuzindikira mndandanda SPE

Chigawo chapadera chodziwira utoto wa Azo: sankhani chodzaza kwambiri cha diatomite; Ukadaulo wapadera wa sieve-plate flow rate control, kudzera pakuyezetsa kwanyumba ndi akunja ovomerezeka, OEM supply.

Komanso, pali graphitized mpweya wakuda, asidi alkali neutral aluminiyamu, kokonati chipolopolo adamulowetsa mpweya, uchi kudziwika ndime, chikopa decolorization column, plasticizer kudziwika ndime ...... SPE column, more SPE mankhwala chonde funsani.

Masitepe (tengani Malangizo a SPE monga chitsanzo):

Opaleshoniyo ndi yosiyana pang'ono kutengera momwe sorbent imasungidwira ndi ma adsorption a zinthu zomwe mukufuna kapena zonyansa.

1. The sorbent adsorbs chandamale mankhwala, kuchotsa zonyansa ndi kuthawa chandamale mankhwala

Mfundo Yofunika:

kutsitsa zitsanzo kuyeretsa, kukana kutsuka, elution

Mtundu uwu wa olimba gawo m'zigawo ntchito zambiri ali ndi njira zinayi:

(1) kutsegula - kuchotsa zonyansa mu Tip SPE ndi kubadwa kwa malo ena osungunulira;

(2) katundu chitsanzo - Sungunulani chitsanzo mu zosungunulira zina, ntchito pipette mpweya Tip SPE ndi kusunga zigawo zikuluzikulu pa Tip;

(3) leaching - kuchotsedwa kwakukulu kwa zonyansa ndi zinthu zina zomwe sizinapangidwe;

(4) elution - elution ndi kusonkhanitsa chandamale mankhwala ndi buku laling'ono zosungunulira.

2. The sorbent adsorbs zonyansa ndi kuchotsa chandamale mankhwala

Mfundo Yofunika:

Adsorb zonyansa pachitsanzo ndikuchotsa zomwe mukufuna

Mtundu woterewu wagawo lolimba nthawi zambiri umakhala ndi masitepe atatu:

(1). Kutsegula - chotsani zonyansa mu Tip SPE ndikupanga malo ena osungunulira.

(2) kutsitsa zitsanzo - lowetsani Tip SPE ndi pipette kenako ndikuwuphulitsa pang'onopang'ono. Panthawiyi, mankhwala ambiri omwe akuwunikira adzawomberedwa ndi njira yoyambira, ndipo zonyansa zidzasungidwa pa Tip. Choncho, masitepe ayenera kuyamba kusonkhanitsa.

(3) Elution - eluting zigawo zikuluzikulu ndi buku laling'ono zosungunulira ndi kusonkhanitsa, kaphatikizidwe zosonkhanitsira madzi.

Zambiri kapena makonda anu, mwalandilidwamakasitomala onse atsopano ndi akale kufunsa, kukambirana mgwirizano, kufunafuna chitukuko wamba!