Oligonucleotide (Oligonucleotide), nthawi zambiri amatanthauza kachigawo kakang'ono ka polynucleotide ka 2-10 nucleotide zotsalira zolumikizidwa ndi phosphodiester bond, koma mawuwa akagwiritsidwa ntchito, kuchuluka kwa nucleotide zotsalira Palibe malamulo okhwima. M'mabuku ambiri, mamolekyu a polynucleotide okhala ndi zotsalira za nucleotide 30 kapena kupitilira apo amatchedwa oligonucleotides. Oligonucleotides amatha kupangidwa ndi zida. Atha kugwiritsidwa ntchito ngati ma DNA synthesis primers, ma gene probes, ndi zina zambiri, ndipo amakhala ndi ntchito zambiri pakufufuza zamakono zamamolekyulu.

ntchito

Oligonucleotides amagwiritsidwa ntchito ngati ma probes kuti adziwe kapangidwe ka DNA kapena RNA, ndipo amagwiritsidwa ntchito munjira monga gene chip, electrophoresis, ndi fluorescence in situ hybridization.

DNA yopangidwa ndi oligonucleotide ingagwiritsidwe ntchito pamayendedwe a polymerization, omwe amatha kukulitsa ndikutsimikizira pafupifupi zidutswa zonse za DNA. Pochita izi, oligonucleotide imagwiritsidwa ntchito ngati choyambira kuphatikiza ndi chidutswa cholembedwa chothandizira mu DNA kupanga kopi ya DNA. .

Oligonucleotides olamulira amagwiritsidwa ntchito poletsa zidutswa za RNA ndikuletsa kuti asamasuliridwe kukhala mapuloteni, omwe amatha kuchitapo kanthu poletsa ntchito ya maselo a khansa.