Nucleic acid extractor ndi chida chomwe chimagwiritsa ntchito ma reagents ofananira a nucleic acid kuti amalize kutulutsa kwa nucleic acid. Amagwiritsidwa ntchito kwambiri m'magawo osiyanasiyana monga Center for Disease Control, matenda a chipatala, chitetezo chothiridwa magazi, chizindikiritso chazamalamulo, kuyesa kwachilengedwe kwa tizilombo toyambitsa matenda, kuyezetsa chitetezo chazakudya, kuweta nyama ndi kafukufuku wama cell biology.

1. Amagawidwa molingana ndi kukula kwa chida

1)Makina ogwirira ntchito amadzimadzi

Makina ogwiritsira ntchito madzi amadzimadzi ndi chida champhamvu kwambiri, chomwe chimangomaliza kutulutsa madzi ndi kukhumba, ndipo chimatha kuzindikira zonse zodziwikiratu za kutulutsa kwachitsanzo, kukulitsa, ndi kuzindikira pophatikiza ntchito monga kukulitsa ndi kuzindikira. Nucleic acid m'zigawo ndi ntchito imodzi yokha ntchito yake, ndipo si oyenera chizolowezi labotale m'zigawo za asidi nucleic. Amagwiritsidwa ntchito pazosowa zoyesera za mtundu umodzi wa chitsanzo ndi kuchuluka kwambiri kwa zitsanzo (osachepera 96, kawirikawiri mazana angapo) panthawi imodzi. Kukhazikitsidwa kwa nsanja ndikugwira ntchito kwa malo ogwirira ntchito kumafuna ndalama zambiri.

2)Small automatic nucleic acid extractor

Chida chaching'ono chodziyimira pawokha chimakwaniritsa cholinga chongotulutsa nucleic acid kudzera muzomwe zimapangidwira, ndipo zitha kugwiritsidwa ntchito mu labotale iliyonse.

2. Siyanitsani molingana ndi mfundo yochotsa

1)Zida zogwiritsira ntchito njira ya spin column

Njira ya centrifugal column nucleic acidchopopera makamaka ntchito osakaniza centrifuge ndi basi pipetting chipangizo. Kutulutsa nthawi zambiri kumakhala zitsanzo 1-12. Nthawi yogwira ntchito ndi yofanana ndi yochotsa pamanja. Sichimapangitsa kuti ntchito ikhale yabwino komanso yokwera mtengo. Zitsanzo zosiyana Zomwe zimagwiritsidwa ntchito pazida sizili zapadziko lonse, ndipo ndizoyenera ma laboratories akuluakulu omwe ali ndi ndalama zokwanira.

2) Zida zogwiritsira ntchito njira ya magnetic bead

Kugwiritsa ntchito mikanda maginito monga chonyamulira, ntchito mfundo ya maginito mikanda adsorbing zidulo nucleic pansi mchere mkulu ndi otsika pH makhalidwe, ndi kuwalekanitsa ndi nucleic zidulo pansi mchere wochepa ndi mkulu pH makhalidwe, lonse nucleic asidi m'zigawo ndi kuyeretsa ndondomeko anazindikira ndi kusuntha. maginito mikanda kapena kusamutsa madzi. Chifukwa chapadera cha mfundo yake, ikhoza kupangidwa kuti ikhale yosiyana siyana, yomwe imatha kuchotsedwa ku chubu limodzi kapena ku zitsanzo za 8-96, ndipo ntchito yake ndi yosavuta komanso yachangu. Zimangotenga mphindi 30-45 kuti mutulutse zitsanzo za 96, zomwe zimasintha kwambiri Kuchita bwino kwa kuyesera ndi mtengo wotsika zimalola kuti zigwiritsidwe ntchito m'ma laboratories osiyanasiyana. Pakali pano ndi chida chachikulu pamsika.