Nucleic Acid Extraction System (Nucleic Acid Extraction System) ndi chida chomwe chimagwiritsa ntchito ma reagents ofananira a nucleic acid kuti amalize kutulutsa zitsanzo za nucleic acid. Amagwiritsidwa ntchito kwambiri m'malo owongolera matenda, kuwunika kwa matenda am'chipatala, chitetezo chothiridwa magazi, chizindikiritso chazamalamulo, kuyezetsa tizilombo tating'onoting'ono, kuyesa chitetezo chazakudya, kuweta nyama ndi kafukufuku wama cell biology ndi zina.
1. Njira yoyamwa, yomwe imadziwikanso kuti njira ya pipetting, ndiyo kuchotsa nucleic acid mwa kusuntha mikanda ya maginito ndi kusamutsa madzi. Nthawi zambiri, kusamutsako kumachitika poyang'anira mkono wa robotic kudzera pa opaleshoni. Njira yochotsera ili motere:

1) Lysis: Onjezani njira ya lysis ku chitsanzo, ndikuzindikira kusakanikirana ndi kuchitapo kanthu kwa njira yothetsera njira pogwiritsa ntchito makina ndi kutentha, maselo amapangidwa ndi lysed, ndipo nucleic acid imatulutsidwa.

2) Adsorption: Onjezani mikanda ya maginito ku chitsanzo cha lysate, sakanizani bwino, ndikugwiritsa ntchito mikanda ya maginito kuti mukhale ndi mgwirizano wamphamvu wa nucleic acids pansi pa mchere wambiri ndi pH yochepa kuti mutenge nucleic acid. Pansi pa zochita za maginito akunja, mikanda ya maginito imasiyanitsidwa ndi yankho. , gwiritsani ntchito nsongayo kuchotsa madziwo ndi kuwataya ku thanki ya zinyalala, ndi kutaya nsongayo.

3) Kutsuka: Chotsani mphamvu ya maginito yakunja, sinthani ndi nsonga yatsopano ndikuwonjezera chotchinga chochapira, sakanizani bwino kuti muchotse zonyansa, ndikuchotsa madziwo pansi pa mphamvu ya maginito akunja.

4) Elution: Chotsani mphamvu ya maginito yakunja, m'malo ndi nsonga yatsopano, onjezani zotchingira za elution, sakanizani bwino, kenako mulekanitse nucleic acid yomangidwa ndi mikanda ya maginito kuti mupeze asidi oyeretsedwa.
2. Njira yamagetsi yamagetsi

Njira ya magnetic rod imazindikira kulekanitsa kwa nucleic acid pokonza madzi ndi kusamutsa mikanda ya maginito. Mfundo ndi ndondomeko ndi zofanana ndi za njira yoyamwa, koma kusiyana ndi njira yolekanitsa pakati pa mikanda ya maginito ndi madzi. Njira ya maginito ya bar ndi yolekanitsa mikanda ya maginito kuchokera kumadzi otayira kudzera pakukoka kwa maginito ndodo kupita ku mikanda ya maginito, ndikuyika mumadzi ena kuti muzindikire kutulutsa kwa nucleic acid.