Kulekanitsa ndi kuyeretsedwa kwa mapuloteni kumagwiritsidwa ntchito kwambiri mu kafukufuku wa biochemistry ndi kugwiritsa ntchito ndipo ndi luso lofunika kwambiri. SCG Protein Purification System Company-Saipu Instrument yaphatikiza zolekanitsa komanso kulekanitsa bwino zamapulotenikuyeretsedwa kwa aliyense. Selo lodziwika bwino la eukaryotic limatha kukhala ndi ma protein ambiri osiyanasiyana, ena ndi olemera kwambiri ndipo ena amakhala ndi makope ochepa. Kuti muphunzire puloteni inayake, m'pofunika poyamba kuyeretsa mapuloteni kuchokera ku mapuloteni ena ndi mamolekyu omwe si a mapuloteni.

Kupatukana kolimba

Pamene mapuloteni (nthawi zina osakanikirana ndi nucleic acids, polysaccharides, etc.) akupezeka, njira zoyenerera zimasankhidwa kuti zilekanitse zomwe mukufuna.mapulotenikuchokera ku zonyansa zina. Nthawi zambiri, gawo lolekanitsali limagwiritsa ntchito njira monga salting out, isoelectric point accumulation and organic solvent fractionation. Njirazi zimadziwika ndi kuphweka komanso mphamvu zazikulu zogwirira ntchito, zomwe zimatha kuchotsa zonyansa zambiri ndikuyika puloteni yothetsera. Mapuloteni ena opangidwa ndi kuchuluka kwake ndipo sali oyenera kuyikapo mwa kudziunjikira kapena kutulutsa mchere. Mutha kusankha ultrafiltration, kusefera gel osakaniza, kuzizira vacuum kuyanika kapena njira zina ndende.

Kupatukana kwabwino

Pambuyo pakugawanitsa kwachitsanzo, voliyumu nthawi zambiri imakhala yaying'ono, ndipo zonyansa zambiri zachotsedwa. Kuti muyeretsedwenso, njira za chromatography nthawi zambiri zimaphatikiza kusefera kwa gel, ion exchange chromatography, adsorption chromatography, ndi affinity chromatography. Ngati ndi kotheka, mukhoza kusankha electrophoresis, kuphatikizapo zone electrophoresis, isoelectric point set, etc. monga njira yomaliza yoyeretsa. Njira yomwe imagwiritsidwa ntchito pakulekanitsa magawo ang'onoang'ono nthawi zambiri imakhala yaying'ono pokonzekera, koma yokhala ndi malingaliro apamwamba.

Crystallization ndi njira yomaliza yolekanitsa mapuloteni ndi kuyeretsa. Ngakhale kuti ndondomeko ya crystallization sikuonetsetsa kuti puloteni iyenera kukhala yofanana, ndi pamene puloteni inayake imakhala ndi ubwino mu njira yothetsera kristalo. The crystallization ndondomeko palokha limodzi ndi mlingo winawake wa kuyeretsedwa, ndi recrystallization akhoza kuchotsa pang'ono achinyengo mapuloteni. Kuyambira denaturedmapulotenisichinapezekepo panthawi ya crystallization, mapuloteni a crystallization si chizindikiro cha chiyero, komanso chitsogozo champhamvu chodziwira kuti mankhwalawa ali mu chikhalidwe chake.