Botolo lachitsanzo ndi chidebe chowunikira zida zomwe ziyenera kufufuzidwa, ndipo ukhondo wake umakhudza mwachindunji zotsatira zake. Nkhaniyi ikufotokoza mwachidule njira zosiyanasiyana zoyeretsera botolo lachitsanzo cha chromatographic, ndipo cholinga chake ndi kupereka tanthauzo kwa aliyense. Njirazi zatsimikiziridwa ndi abwenzi ndi oyambirira. Amakhala ndi zotsatira zabwino zotsuka pazotsalira zosungunuka zamafuta ndi zotsalira za organic reagent muchromatography chitsanzo botolo. Ukhondo umakwaniritsa zofunikira, njira zoyeretsera zimakhala zosavuta, ndipo nthawi yoyeretsa imachepetsedwa, ndipo njira yoyeretsera imakhala yabwino kwambiri pa chilengedwe .

Chonde pangani chisankho chanu kutengera momwe muliri mu labotale yanu!

Pakadali pano, ndi chidwi chowonjezeka chazakudya komanso chitetezo chamitundu yonse, ukadaulo wowunikira ma chromatographic ukugwiritsidwa ntchito kwambiri pakuyesa kwazakudya komanso chitetezo, makamaka pakuyesa kwazinthu zaulimi, ukadaulo wowunikira ma chromatographic wagwiritsidwa ntchito kwambiri. M'dziko langa, zinthu zambiri zaulimi (mankhwala ena, ma organic acid, ndi zina zotero) ziyenera kuyesedwa ndi madzi chromatography ndi gas chromatography chaka chilichonse. Chifukwa cha kuchuluka kwa zitsanzo, pali mabotolo ambiri omwe amafunikira kutsukidwa panthawi yozindikira, zomwe sizimangowononga nthawi komanso zimachepetsa kugwira ntchito bwino, komanso nthawi zina zimapangitsa kuti pakhale kusiyana kwa zotsatira zoyesera chifukwa cha ukhondo wa kutsukidwa zitsanzo mabotolo.

Thechromatographic chitsanzo botoloamapangidwa makamaka ndi galasi, kawirikawiri pulasitiki. Mabotolo a zitsanzo zotayidwa ndi okwera mtengo, owononga, ndipo amawononga kwambiri chilengedwe. Ma laboratories ambiri amatsuka mabotolo a zitsanzo ndikuwagwiritsanso ntchito. Pakalipano, njira zomwe zimagwiritsidwa ntchito m'ma laboratories kuyeretsa botolo lachitsanzo makamaka ndikuwonjezera ufa wochapira, zotsukira, zosungunulira za organic, ndi mafuta odzola a asidi, kenaka ndikutsuka ndi chubu choyesera chokhazikika. Njira yoyeretsera imeneyi ili ndi zofooka zambiri. Imagwiritsa ntchito zotsukira ndi madzi ambiri, imatenga nthawi yayitali kuchapa, ndipo imasiya ngodya zakufa. Ngati ndi botolo la pulasitiki, n'zosavuta kusiya zizindikiro za burashi pakhoma la botolo lamkati, zomwe zimatengera anthu ambiri. Kwa magalasi omwe amadetsedwa kwambiri ndi zotsalira za lipid ndi mapuloteni, njira ya alkaline lysis imagwiritsidwa ntchito poyeretsa, ndipo zotsatira zabwino zimapindula.

Posanthula zitsanzo, kuyeretsa botolo la jakisoni ndikofunikira kwambiri. Malinga ndi njira yotsuka magalasi, njira yoyeretsera imasankhidwa molingana ndi kuchuluka kwa kuipitsa, ndipo palibe njira yokhazikika. Chidule cha njira:

1. Thirani njira yoyesera mu botolo louma

2. Imirirani zonse mu 95% mowa, kusamba kawiri ndi akupanga ndi kutsanulira, chifukwa mowa mosavuta kulowa 1.5mL vial ndipo akhoza miscible ndi zambiri organic solvents kukwaniritsa kuyeretsa kwenikweni.

3. Thirani madzi oyera, ndi ultrasonically kusamba kawiri.

4. Thirani mafuta odzola mu botolo louma ndikuphika pa madigiri 110 Celsius kwa maola 1 ~ 2. Osaphika pa kutentha kwakukulu.

5. Kuzizira ndi kusunga.