Blot Analysis mu Biopharmaceutical, Medical ndi Magawo Ena

“Mapulani a Zaka Zisanu za Zaka 14” akulimbikitsa kuti chuma cha bioeconomy chiziyenda bwino chifukwa cha chitukuko ndi kupita patsogolo kwa sayansi ya zamoyo ndi sayansi ya sayansi ya zamoyo, potengera kuteteza, chitukuko ndi kagwiritsidwe ntchito ka zinthu zachilengedwe, komanso kutengera kusakanikirana kwakukulu komanso kozama kwa sayansi. mankhwala, thanzi, ulimi, nkhalango, ndi mphamvu. , kuteteza chilengedwe, zipangizo ndi mafakitale ena; zikuwonekeratu kuti chitukuko cha bio-economy ndi njira yofunikira kuti igwirizane ndi kusintha kwachangu kwa sayansi ya sayansi ya zamoyo padziko lonse ndi kukwaniritsa kudzidalira kwapamwamba pa sayansi ndi luso lazopangapanga. Ndi gawo lofunikira kulima ndikukulitsa bizinesi ya bio ndikulimbikitsa chitukuko chachuma chapamwamba. Kukwaniritsa kukula kwachangu kwa moyo ndi zosowa zaumoyo ndikukwaniritsa chikhumbo cha anthu chokhala ndi moyo wabwino ndi chitsimikizo chofunikira kulimbikitsa chitetezo ndi kuwongolera kwachitetezo cha chitetezo cha dziko komanso kulimbikitsa kutsogoza kwa kayendetsedwe ka kayendetsedwe ka kayendetsedwe kake ndi kayendetsedwe ka dziko.

Poyankha kuyitanidwa kwa dziko, BM yadzipereka kugonjetsa ukadaulo wapamwamba wopanga mafilimu ndikuzindikira pang'onopang'ono kulowetsa m'malo mwa zinthu zamtengo wapatali m'munda wa sayansi ya moyo. Mu Meyi 2023, kupanga kwakukulu kwa immunochromatography NCmembranes idakwaniritsidwa bwino ndikuyika kuzinthu zosiyanasiyana zozindikira mwachangu. Pakadali pano, filimu ya NC yakhala ikugwiritsidwa ntchito pakuwunika kwapanyumba mu m'galasi, chitetezo chazakudya, kuyezetsa mwachangu mankhwala ndi magawo ena, ndipo yakwanitsa kutumiza kunja ndikupikisana ndi zimphona zapadziko lonse lapansi pamsika! Titamaliza zokambirana za msika wa filimu wa NC, patatha miyezi ingapo ya kafukufuku waukadaulo wopangidwa ndi gulu lathu laukadaulo, poyankha zofunikira zachangu za ogwiritsa ntchito padziko lonse lapansi sayansi ya moyo wapadziko lonse lapansi kuti achepetse mtengo wazinthu zamtengo wapatali zogulira, tinayambitsa bwino kufufutitsa.membranes, yomwe ili yoyenera kwa biopharmaceuticals, mankhwala ndi zina. Western blotting analysis (Western Blotting, WB)

Chiyambi cha mawonekedwe a BM Blotting Membranes, : Kukula kwa pore ndi mtundu wa mapuloteni oyenerera 0.1μm oyenera mapuloteni okhala ndi maselo olemera osakwana 7kDa 0.22μm oyenera mapuloteni okhala ndi maselo olemera osakwana 20kDa 0.45μm oyenera mapuloteni okhala ndi mamolekyulu olemera kuposa 20kDa Main Mfundo zomangira mapuloteni Magetsi osasunthika ndi hydrophobicity Mkhalidwe wogwiritsiridwa ntchito wosinthira ndi njira zozindikirira Chemiluminescence Fluorescence kuzindikira kwa radiolabeled probe mwachindunji kudaya ma antibodies olumikizidwa ndi enzyme Ubwino:

1.Low background, high sensitivity

2.No kufunika mowa reagent chisanadze wetting

3.Mapangidwe apadera a pamwamba ndi katundu amapanga chiŵerengero chabwino kwambiri cha chizindikiro ndi phokoso Zomwe zimachokera ku ulusi wachilengedwe, ndizogwirizana ndi chilengedwe, ndipo zimatha kusunga mapuloteni omangika kwa nthawi yaitali.

Chiyambi cha ukadaulo wowunikira wa WB Ukadaulo wowunika wa WB ndiukadaulo womwe umagwiritsidwa ntchito kwambiri pazasayansi yama cell biology, biochemistry, immunology ndi zina. Tekinoloje iyi imagwiritsa ntchito kumangiriza kwa ma antibodies ku mapuloteni enieni mu minofu kapena ma cell kuti akwaniritse chizindikiritso cha mapuloteni ndi kusanthula mawu potengera malo ndi kulimba kwa gulu lamtundu, ndiko kuti, kusanthula kwamtundu ndi theka-quantitative. Choyamba chinaperekedwa ndi Harry Towbin wa Friedrich Miescher Institute ku Switzerland mu 1979. Zakhala zaka zoposa 40 zapitazo ndipo yakhala njira yapamwamba kwambiri komanso yothandiza yofufuza mapuloteni.