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BM Life Science，Ultrafiltration Centrifugal Tube (Centrifugal Filter) Series Products
It consists of an inner filter tube (with membrane) + an outer centrifuge tube, which can quickly and effectively purify and concentrate up to 15ml of biological samples. The unique vertical design and maximized filtration area can provide fast sample processing capacity and high sample recovery rate (usually ≥90% dilute original solution), while maintaining a mild filtration and concentration environment to maintain protein activity and Conformation.The unique vertical design can polarize the solute and significantly reduce the fouling of the filter membrane caused by subsequent centrifugation. By optimizing the physical filter stop point in the filter device, it can prevent the centrifugal filter from drying out the sample or causing sample loss due to excessive rotation. . A variety of ultrafiltration molecular weight cutoff (MWCO) centrifugal filters use high-purity, low-adsorption PES and RCE membranes, which have extremely low protein and nucleic acid binding rates.
BM Life Science, clean room production in all links of Ultrafiltration Centrifugal Tube (Centrifugal Filter), assembly line operation, full ERP management, ultra-pure products, no DNase/RNase, no PCR inhibitors, no heat source. BM Life Science, all sizes of Ultrafiltration Centrifugal Tube (Centrifugal Filter) series products are customized, the series of products are stable in batches with minimal difference between batches and high in quality. They can be replaced by domestic products and exported to Japan, South Korea, Europe, and the United States. Widely used in conventional the sample separation and filtration experiments.
Application
1 Ultrafiltration Buffer replacement, purification, concentration, and desalination of antigens, antibodies, enzymes, and other proteins, as well as biological samples such as nucleic acids and microorganisms, desalination of chromatographic components, separation of biological molecules from tissue culture or cell lysis solutions, removal of primers, connectors, or molecular markers from reaction mixtures, virus concentration or separation, and crude separation of biological molecule mixtures.
2 Microfiltration separates nucleic acids and proteins in gels, samples are filtered and clarified, and processed particles or beads are collected and washed.
Feature of Product
★ High concentration ratio, which can easily reach 80-100 times concentration ratio；
★ The sample concentration speed is fast, and the general concentration time is 10-60 minutes. It also has an anti-drying lock design to avoid damage to the sample due to excessive centrifugation；
★ The sample recovery rate is high and can reach a recovery rate of more than 90%；
★ Low adsorption, PES/RC membrane and smooth inner wall design have extremely low protein adsorption and biomolecule binding rate；
4ml/15ml、15ml/50ml
★ Complete specifications, ultrafiltration centrifuge tubes with two volumes of 4ml/15ml and 15ml/50ml, with various pore sizes, available in 3KD, 5KD, 10KD, 30KD, 50KD and 100KD to meet different molecular weight cutoff requirements；
★ Convenient operation, the entire separation and filtration process can be completed with a Multifunctional Centrifuge, easy to operate；
★ The product is clean, all links are produced in a clean room, assembly line operation, full ERP management, the product is ultra-pure, no DNase/RNase, no PCR inhibitor, no heat source;
★ High definition, no background and auto-fluorescence, and does not affect the detection results;
★ Reliable performance, good sealing, and does not react with the experimental samples, the experimental results are reliable, and can withstand high temperatures of 100 ℃!
★ It has a wide range of applications. For different applications, two types of Ultrafiltration Centrifugal Tube (Centrifugal Filter) series products, 4ml/15ml and 15ml/50ml are introduced to meet different customer needs, accepting customization and functional development!
Order Information
| Cat.No | Name | Specification | Description | Pcs/pk |
| UCT015RCE004003 | Ultrafiltration Centrifugal Tube | 4/15ml，3kD | ，RCE Membranes | 15 pcs/bag |
| UCT015RCE004010 | Ultrafiltration Centrifugal Tube | 4/15ml，10kD | RCE Membranes | 15 pcs/bag |
| UCT015RCE004030 | Ultrafiltration Centrifugal Tube | 4/15ml，30kD | RCE Membranes | 15 pcs/bag |
| UCT015RCE004100 | Ultrafiltration Centrifugal Tube | 4/15ml，100kD | RCE Membranes | 15 pcs/bag |
| UCT050RCE015003 | Ultrafiltration Centrifugal Tube | 15/50ml，3kD | RCE Membranes | 24 pcs/bag |
| UCT050RCE015010 | Ultrafiltration Centrifugal Tube | 15/50ml，10kD | RCE Membranes | 24 pcs/bag |
| UCT050RCE015030 | Ultrafiltration Centrifugal Tube | 15/50ml，30kD | RCE Membranes | 24 pcs/bag |
| UCT050RCE015100 | Ultrafiltration Centrifugal Tube | 15/50ml，100kD | RCE Membranes | 24 pcs/bag |
| UCT050PES015005 | Ultrafiltration Centrifugal Tube | 15/50ml，5kD | PES Membranes | 12 pcs/bag |
| UCT050PES015010 | Ultrafiltration Centrifugal Tube | 15/50ml，10kD | PES Membranes | 12 pcs/bag |
| UCT050PES015030 | Ultrafiltration Centrifugal Tube | 15/50ml，30kD | PES Membranes | 12 pcs/bag |
| UCT050PES015050 | Ultrafiltration Centrifugal Tube | 15/50ml，50kD | PES Membranes | 12 pcs/bag |
| UCT050PES015100 | Ultrafiltration Centrifugal Tube | 15/50ml，100kD | PES Membranes | 12 pcs/bag |
| CF050PES015C010 | Centrifugal Filter | 15/50ml，0.1um | 0.1um，PES Membranes | 12 pcs/bag |
| CF050PES015C020 | Centrifugal Filter | 15/50ml，0.2um | 0.2um，PES Membranes | 12 pcs/bag |
| CF050PES015C045 | Centrifugal Filter | 15/50ml，0.45um | 0.45um，PES Membranes | 12 pcs/bag |
| UCT** | Ultrafiltration Centrifugal Tube | Customizing | Customizing | ** pcs/bag |
| CF** | Centrifugal Filter | Customizing | Customizing | ** pcs/bag |
BM Life Sciences, Ultrafiltration Centrifugal Tube (Centrifugal Filter) series product parameters and experimental data
Ultrafiltration Centrifugal Tube (Centrifugal Filter) series product performance and experimental data
Ultrafiltration Centrifugal of samples:
5 KD 0.25mg/ml Cyt C；
10 KD1mg/ml OVA；
30/50 KD 1mg/ml BSA；
100 KD 1mg/ml IgG。
Experimental conditions : horizontal rotor, centrifugal force 4000×g , 8℃, starting volume 15ml.
Ultrafiltration Centrifugal of samples:
5 KD 0.25mg/ml Cyt C；
10 KD 1mg/ml OVA；
30/50 KD 1mg/ml BSA；
100 KD 1mg/ml IgG.
Experimental conditions : fixed angle rotor, centrifugal force 5000×g , 8℃, starting volume 12ml.
Typical rejection rates for protein molecular weight standards (4000g x 15ml starting volume for rocking rotor or 5000g x 12ml starting volume for fixed angle rotor)
| molecular weight standards/concentration | molecular weight | NMWL Ultrafiltration tube | rejection rates % Set the bucket | Rejection rates % fixed angle | centrifugation time |
| B-12 vitamin | 1350 | 3K | ＜25 | ＜25 | 60 |
| （0.25mg/ml）Cytochrome C | 12400 | ＞95 | ＞95 | 60 | |
| （1mg/ml）α-Chymotrypsinogen | 25000 | ＞95 | ＞95 | 60 | |
| B-12（0.2mg/ml） vitamin | 1350 | 10K | ＜5 | ＜5 | 30 |
| 0.25mg/ml）Cytochrome C | 12400 | ＞95 | ＞95 | 30 | |
| （1mg/ml）α-Chymotrypsinogen | 25000 | ＞95 | ＞95 | 30 | |
| vitamin B-12（0.2mg/ml） | 1350 | 30K | ＜5 | ＜5 | 20 |
| Cytochrome C 0.25mg/ml） | 12400 | ＜10 | ＜10 | 20 | |
| Ovalbumin（1mg/ml） | 45000 | ＞95 | ＞95 | 20 | |
| BSA（1mg/ml） | 67000 | ＞95 | ＞95 | 20 | |
| Cytochrome C（0.25mg/ml） | 12400 | 50K | ＜5 | ＜5 | 10 |
| Ovalbumin（1mg/ml） | 45000 | 65 | 55 | 10 | |
| BSA（1mg/ml） | 67000 | ＞90 | ＞90 | 10 | |
| Ovalbumin（1mg/ml | 45000 | 100K | ＜25 | ＜15 | 20 |
| IgG（1mg/ml） | 156000 | ＞90 | ＞90 | 20 | |
| Thyroglobulin（0.5mg/ml） | 677000 | ＞90 | ＞90 | 20 |
Recovery rate
The retention capacity of the 15ml ultrafiltration centrifuge tube membrane is described by the molecular weight cutoff (MWCO).
Solute molecular weights close to MWCO may only be partially retained. In order to obtain the best retention characteristics,
please use a MWCO filter membrane with a molecular weight of 1/3 or less than the solute.
Product recovery rate comparison
Comparison of protein recovery rates and dead volumes of different brands of ultrafiltration tubes
|
10KD Comparison of protein recovery rates and dead volumes of different brands of ultrafiltration tubes |
|||
| Brand | （%）Recovery rate | （μl）Dead volume | |
| BM | 1㎎/ml Protein concentration | 90 | 87（Protein concentration1㎎/ml） |
| 0.1㎎/ml Protein concentration | 83 | ||
| Brand M | 1㎎/ml Protein concentration | 93 | 47（Protein concentration1㎎/ml） |
| 0.1㎎/ml Protein concentration | 88 | ||
| Brand S | 1㎎/ml Protein concentration | 91 | 70（Protein concentration1㎎/ml） |
| 0.1㎎/ml Protein concentration | 85 | ||
| Brand K | 1㎎/ml Protein concentration | 89 | 186（Protein concentration1㎎/ml） |
| 0.1㎎/ml Protein concentration | 77 | ||
| Brand J | 1㎎/ml Protein concentration | 83 | |
| 0.1㎎/ml Protein concentration | 50 | ||
| Experimental conditions: OVA protein (molecular weight 45KD), horizontal rotor 4000g | |||
Reference selection specifications for ultrafiltration tubes with different molecular weights
| starting volume | ＜0.5ml | ＜2ml | ＜4ml | ＜15ml | |
| Protein | |||||
| (MW)molecular weight | MWCO (Da) molecular weight cutoff | ||||
| 6K＜MW＜20K | 3000 | 3000 | 3000 | 3000 | |
| 20K＜MW＜60K | 10000 | 10000 | 10000 | 10000 | |
| 60K＜MW＜100K | 30000 | 30000 | 30000 | 30000 | |
| 100K＜MW＜200K | 50000 | 50000 | 50000 | 50000 | |
| 200K＜MW | 100000 | 100000 | 100000 | 100000 | |
| Single-stranded and double-stranded nucleic acids | |||||
| length | MWCO (Da) molecular weight cutoff | ||||
| 137-1159bp | 3000 | 3000 | 3000 | 3000 | |
| nanoparticles | |||||
| （DIA）particle size | MWCO (Da) molecular weight cutoff | ||||
| 1.5＜DIA＜3nm | 3000 | 3000 | 3000 | 3000 | |
| 3＜DIA＜5nm | 10000 | 10000 | 10000 | 10000 | |
| 5＜DIA＜7nm | 30000 | 30000 | 30000 | 30000 | |
| 7＜DIA＜10nm | 50000 | 50000 | 50000 | 50000 | |
| 10 nm＜DIA | 100000 | 100000 | 100000 | 100000 | |
Post time: Feb-18-2024
