Ogidi & Efere Ebugoro Ni-NTA

①Ngwa ngwaahịa zuru ezu

Otu ngwaahịa: kọlụm Ni-NTA/efere maka nkwadebe ihe nlere anya protin peptide na ịdị ọcha protein

Ihe: PP + Agarose gel

Olu nke kọlụm: 1ml, 3ml, 5ml, 6ml, 12ml na 2ml 96-oghere desalination na efere dị ọcha.

Ọrụ: mmịpụta siri ike nke protein polypeptide antibody, filtration, adsorption, nkewa, mmịpụta, ịdị ọcha na itinye uche nke ihe atụ lekwasịrị anya.

Nkọwapụta: 0.5ml/1ml, 1ml/3ml, 1.5ml/3ml, 1.5ml/5ml, 2.5ml/5ml, 2ml/6ml, 3ml/6ml, 4ml/12ml, 6ml/12ml, 0.4ml×96,0.8ml×96

Nkwakọ ngwaahịa: 25 iberibe / 1ml, 20 iberibe / 3ml, 30 iberibe / 6ml, 20 iberibe / 12ml, 5 iberibe / igbe nke ọkara chromatography kọlụm, 1 ibe / mkpọ nke 96-oghere desalination na ọcha efere.

Nkwakọ ngwaahịa: akpa foil aluminum ma ọ bụ opaque aluminum foil bag (nhọrọ)

Igbe nkwakọ ngwaahịa: igbe akara na-anọpụ iche ma ọ bụ igbe agba BML (nhọrọ)

Omenala LOGO: ee

ụdị ọkọnọ: OEM/ODM

②Nkọwa ngwaahịa

BM ndụ sayensị Ni-NTA kọlụm e ọcha site protein affinity chromatography na agarose dị ka crosslinking medium.The ịdị ọcha ụkpụrụ bụ ọcha lekwasịrị protein na mkpado site na iji kpọmkwem adịgide adịgide onwunwe n'etiti nickel ions chelated on NTA na tag.This usoro dị mfe iji rụọ ọrụ. , nnukwu ego nke ime ka ọ dị ọcha, dị mfe iji mee ka protein dị ọcha dị ọcha.

Nickel chloride na kọlụm Ni nwere ike jikọta ya na protein ya na akara ya (histone). Mgbe ejikọta, eke lekwasịrị anya protein nwere ike nweta site imidazole elution.Packing parameters nke Ni kọlụm na narị mile ndụ sayensị.

Mkpụrụ: 4% ma ọ bụ 6% agarose jikọtara ya;

Nkezi urughuru nha: 90um (45-165um); Ngwọta nchekwa: 20% ethanol ngwọta;

Nkwụsi ike PH: 2-12;

Ndị na-eguzogide ọgwụ: 6M guanidine hydrochloride na 8M urea;

Nagide nrụgide: <0.3mpa, 0.1mpa na-atụ aro;

Adsorption:> 20mg (27kDa) protein / ml;

Ọnọdụ nchekwa: 2-8℃.

BM Life Science Ni preloaded kọlụm na-enye ngwaahịa nke 5 nkọwa, gụnyere 1, 3, 5, 6 na 12ml. 1ml na 5ml bụ kọlụm eburula ibu nke chromatography nke ọkara, nke nwere ike iji uru nke usoro ịdị ọcha nke usoro mmiri mmiri na-eme ka ọ dị ọcha maka ịdị ọcha na ịdị ọcha nke macromolecules.

③Njirimara ngwaahịa:

★nkọwa dị iche iche: 1/3/6/12ml maka sirinji, 1/5ml maka ọkara chromatography kọlụm;

★nrụgide nnabata dị elu: chromatography nke ọkara nke etinyere na kọlụm nkwado nkwado ruo 0.6mpa (6 mmanya, 87 psi);

★mfe iji: ruer interface, ike ga-eji na usoro dịkwuo sample loading, nwekwara ike jikọọ sirinji na peristaltic mgbapụta, nwekwara ike jikọọ kpọmkwem KTA, Agilent, Shimadzu, Mmiri na ndị ọzọ mmiri mmiri na-adọ ọcha usoro;

★A na-ejikarị ya eme ihe: nucleic acid sachara, ọgwụ mgbochi, protein akpọrọ, protein desalination.

Order Ozi

Nkọwa nkọwa agba Cat Mba (ml) mbukota

PNI001-1 red/green NI ọkara mgbali chromatography arụnyere na kọlụm 1 5 / igbe.

PNI001-2 transperent tube/acha uhie uhie mkpuchi NI ụdị sirinji ebugoro kọlụm 1 25 / igbe.

PNI003-1 transperent tube/acha uhie uhie mkpuchi NI ụdị sirinji kọlụm eburula ụzọ 3 20 / igbe.

PNI005-1 red/green NI ọkara chromatography arụnyere na kọlụm 5 5 / igbe.

PNI006-1 transperent tube/okpu uhie NI ụdị sirinji kọlụm eburula ụzọ 6 30 / igbe.

PNI012-1 transperent tube/mkpuchi uhie NI ụdị sirinji kọlụm eburula ụzọ 12 20 / igbe.

BM Life Science, niile usoro nke SPE kọlụm tubes na-mere nke ọgwụ-ọkwa polypropylene injection ịkpụzi;The sieve efere na sintered na ultra-ọcha ultra-high molecular arọ polyethylene.sorbents zụrụ n'ụwa nile, na site ikike nwale, àgwà bụ ntụkwasị obi;10,000 mmepụta ogbako dị ọcha ọkwa, usoro mmepụta ahaziri ahazi, njikwa ERP, njirimara ngwaahịa ngwaahịa; nkọwa ngwaahịa dị iche iche iji gboo mkpa ndị ahịa; ngwaahịa anyị niile bụ ahaziri iji mee ka ndị ahịa nwee ike ịnụ ụtọ ọrụ otu nkwụsị.

Akụkụ nke ngwaahịa usoro BM Life Science SPE

Uru teknụzụ:

★Ụfọdụ SPE sorbents nyocha na mmepe na teknụzụ mmepụta na ikike ikike ọgụgụ isi nke onwe, SPE loading (nkesa ntụ ntụ, ntinye, nkwakọ) bụ akpaaka zuru oke.

★N'ịdabere na uru pụrụ iche nke ụlọ ọrụ ịgbatị ebu CNC injection ịkpụzi ụlọ ọrụ na pearl osimiri delta, njikọta na iji akụrụngwa nke ọma amụbaala ikike mmepụta nke ọkpọkọ kọlụm SPE okpukpu abụọ, belata ọnụ ahịa ebu n'ichepụta na ịkpụzi injection, ma mee ka ọ dịkwuo mma. àgwà ngwaahịa

★Ụlọ ọrụ ahụ nwere ultrafine pụrụ iche na teknụzụ nkesa ntụ ntụ dị mma, nkesa ntụ ntụ kpamkpam na akpaka, batchable, ụzọ scalable iji hụ na ogbe ngwaahịa kwụsiri ike.

★Ọtụtụ narị nde R&D na ikike mmepụta maka efere sieve na isi nzacha ebelatala ọnụ ahịa mmepụta nke SPE ruo ọkwa kacha ala.

★SPE sieve efere bụ kpam kpam, ya dayameta, ọkpụrụkpụ, oghere size nwere ike họrọ na collocated na ọchịchọ.

★ụlọ ọrụ ahụ na-etinye aka dị ukwuu maka mmepụta nkà na ụzụ na ọganihu na-aga n'ihu, karịsịa Tip SPE, sieves free panel inlaying SPE, 96-384-hole plate SPE na nnwere onwe ikike ikike ọgụgụ isi, nke na-ejupụta oghere na China wee ruo ọkwa ọkwa ụwa, na-atụgharị uche. Uru pụrụ iche nke B&M Life Science na mpaghara SPE.

Uru ngwaahịa:

★Ọ dị mfe iji rụọ ọrụ, n'okpuru ọrụ nke ike ndọda nwere ike nweta oke ọsọ ọsọ yana ezigbo nrụpụta

★Ọ nwere ike ịchekwa ọnụ ahịa akụrụngwa na ihe oriri nke ukwuu na-enweghị SPE na akụrụngwa oghere

★sorbent dị ọcha na-enweghị nnyonye anya site na oghere oghere

★elu recycle ọnụego nke 10 ~ 100ppm bụ na kasị mma nso nke 95% ~ 105%

★N'iji ikike mgbasa ozi buru ibu, ọ dị elu karịa ụdị kọlụm SPE ndị ọzọ na China

★Ogo ngwaahịa kwụsiri ike, nrụpụta dị mma, nrụpụta ụkpụrụ ọkọlọtọ (RSD) <5%

★Adịghị atụ egwu kọlụm akọrọ. Akọrọ na mmiri mmiri na-asọpụta bụ otu n'ime oke njehie, ọdịiche ọkọlọtọ (RSD) <0.05%

★ngwaahịa anyị zuru oke n'otu ọkwa dị ka Waters / Agilent / Supelco na ụlọ ọrụ ndị ọzọ

★Ọnụego arụmọrụ nke ngwaahịa anyị so na ndị kacha mma n'ụwa

Ngwa:

★A na-eji ya eme ihe n'ala; Mmanụ; Mmiri nke anụ ahụ (plasma / mmamịrị, wdg); nri na ihe ndị ọzọ.

Nkwenye mma:

★Gbaa mbọ hụ na ngwaahịa ọ bụla tozuru oke, na-agbaso ụkpụrụ njikwa mma siri ike, mejuputa nyocha nke ogbe

★Gbaa mbọ hụ na ngwaahịa ọ bụla enweghị nnyonye anya oghere, ọnụego mgbake dị mma karịa ụkpụrụ mba, iji ruo ọkwa kachasị elu nke ngwaahịa ndị yiri ya.

Nkwa na arụmọrụ:

★Nye nkwado teknuzu ọkachamara n'efu

Nhazi nke kọlụm SPE

Polymer matrix usoro SPE

Na spherical polymer dị ka adsorbent, sorbent urughuru size bụ ọzọ edo, SPE kọlụm eruba ọnụego bụ ihe kwụsiri ike, nke bụ oge gboo polymer kọlụm na laabu, dum usoro nke ngwaahịa e ọtụtụ-eji nri ule. Ngwaahịa dabara na Waters HLBS, MAX's na MCX's.

Silica gel matrix usoro SPE

Omuma silica gel matrix SPE kọlụm, amorphous / sppherical adsorbent, na-arụ ọrụ dị elu na ezigbo ahụmịhe onye ọrụ site n'aka ndị ahịa ntụkwasị obi. A na-ejikarị ngwaahịa na nyocha kemịkalụ.

NDỤMỌDỤ mmịpụta / ịdị ọcha / itinye uche nke SPE

ọ bụ ube pipetting ngwaọrụ na-arụ ọrụ na-etinyere maka wepụ / ọcha enrichment ndu samples ma ọ bụ N'ọnụ nke SPE, ebumnobi ngwaahịa pipetting ube top tinye ụfọdụ C4 / C18 / silicon ntụ ntụ / magnetik beads / ProtingA (G) agarose gel sorbent, eji maka primer/genomic DNA plasmid PCR ngwaahịa/protein peptide/ọgwụ mgbochi iji lekwasịrị anya ngwaahịa / nzacha / mmịpụta / desalting / sachaa / ịba ụba.

96/384-oghere efere usoro SPE

The 96/384-ọma efere efere SPE mepụtara kpọmkwem maka elu-throughput sample pretreatment.Mezue sample tupu nhazi usoro na ụlọ ọrụ si orifice iyo ma ọ bụ akpaka workstation.

Usoro nchọpụta pụrụ iche SPE

Kọlụm nchọpụta pụrụ iche Azo dye: họrọ ultra-dị ọcha diatomite filler; Teknụzụ njikwa ọnụọgụ siri ike pụrụ iche, site na nnwale ụlọ na nke mba ofesi, ọkọnọ OEM.

Na mgbakwunye, e nwere graphitized carbon ojii, acid alkali na-anọpụ iche alumina, aki oyibo shei rụọ ọrụ carbon, mmanụ aṅụ na kọlụm, akpụkpọ anụ decolorization kọlụm, plasticizer nchọpụta kọlụm……SPE kọlụm, ọzọ SPE ngwaahịa biko jụọ.

Nzọụkwụ (were ndụmọdụ SPE dị ka ọmụmaatụ):

Ọrụ ahụ dịtụ iche dabere na usoro njigide nke sorbent na mgbasa ozi nke ngwaahịa ma ọ bụ adịghị ọcha

1. The sorbent adsorbs ngwaahịa ezubere iche, na-ewepụ adịghị ọcha ma gbanarị ngwaahịa ezubere iche

Ụkpụrụ:

loading sample nhicha, jụrụ ịsacha, elution

Ụdị ọrụ mmịpụta oge siri ike a nwere usoro anọ:

(1) ịgbalite - mwepụ nke adịghị ọcha na NDỤMỌDỤ SPE na ọgbọ nke ụfọdụ ihe mgbaze gburugburu ebe obibi;

(2) ibu sample - igbari sample na a ụfọdụ ihe mgbaze, na-eji pipette iku ume NDỤMỌDỤ SPE na-edebe ihe ndị ahụ na NDỤMỌDỤ;

(3) leaching - kacha mwepụ nke adịghị ọcha na ngwaahịa ndị ọzọ na-abụghị ebumnuche;

(4) elution - elution na nchịkọta nke ezubere iche ngwaahịa na obere olu nke mgbaze.

2. The sorbent adsorbs adịghị ọcha ma na-ewepu ezubere iche ngwaahịa

Ụkpụrụ:

Tinye ihe ndị na-adịghị ọcha na nlele ahụ wee chụpụ ngwaahịa ezubere iche

Ụdị mmịpụta oge siri ike a na-enwekarị usoro atọ:

(1). Nkwalite - wepụ ihe adịghị ọcha na ndụmọdụ SPE wee mepụta ụfọdụ ebe mgbaze.

(2) sample loading — kukuba Tip SPE na pipette wee jiri nwayọ fepụ ya. N'oge a, ọtụtụ n'ime ogige ndị e lekwasịrị anya ka a ga-eji ihe nrụnye ihe nrịba ama, a ga-edobekwa adịghị ọcha na Ntụpọ. Ya mere, ekwesịrị ịmalite usoro ndị a na-anakọta.

(3) Elution - eluting components na obere olu nke ihe mgbaze na ịnakọta, na-ejikọta mkpokọta mmiri mmiri.

Nkọwa ndị ọzọ ma ọ bụ ahaziri ahaziri onwe, nnabatandị ahịa ọhụrụ na ndị agadi ka ha jụọ, kparịta imekọ ihe ọnụ, chọọ mmepe nkịtị!