Gịnị bụ usoro ọcha nkeusoro ọcha nke protein? Ọ dị mkpa ịmara usoro ntinye DNA nke protein ahụ emechara, iji hụ mkpụrụ ndụ ma ọ bụ anụ ahụ nke egosipụtara karịa na mkpụrụ ndụ ihe mgbaru ọsọ, na imepụta mkpụrụ ndụ ihe nketa iji bulie akụkụ nke iberibe DNA e lekwasịrị anya. Nke a bụ ihe a na-akpọ inweta iberibe mkpụrụ ndụ ihe nketa.

Mwube nke okwu vector: Na prokaryotic ma ọ bụ eukaryotic okwu vector nwere njirimara nke mkpụrụ ndụ ihe nketa enwetara n'onwe ya, isi nsogbu nke usoro a bụ iwulite plasmid na mkpụrụ ndụ mmasị, na usoro okwu. Oge okwu prokaryotic dị mkpụmkpụ, ọnụ ahịa dị ala, na ọnụ ọgụgụ buru ibu nke okwu bụ ihe kacha mkpa; A naghị egosipụta mkpụrụ ndụ ihe nketa na E. coli, nsogbu ahụ na-eme na njikarịcha codon. N'ịtụle ọrụ ka mma na ịdị ọcha nke protein, ndị nchọpụta ahụ họọrọ ikwupụta na yist Pichi. Ngosipụta nke ọma nke njikarịcha codon dị mkpa.

Kedu usoro nchacha nke sistemu ọcha protein:

1. Mmiri ozuzo.

2. Electrophoresis: Protein ebubo dị elu ma ọ bụ dị ala karịa ebe isoelectric ya ma nwee ike ịkwaga na electrode na-adịghị mma ma ọ bụ electrode ziri ezi nke ọkụ eletrik na mpaghara eletrik. Nkwado ihe nkiri electrophoresis, electrophoresis, wdg.

3. Dialysis: Usoro nke na-eji akpa dialysis abụọ kewapụ nnukwu ụmụ irighiri ihe na protein na obere ogige molecular.

4. Chromatography: ion mgbanwe chromatography na-eji free Njirimara nke protein. N'okpuru pH a kapịrị ọnụ, ụgwọ na akụrụngwa nke protein dị iche iche, enwere ike kewaa ha site na ion mgbanwe chromatography. Na chromatography mgbanwe anion, protein ndị nwere ike adịghị mma na-ebu ụzọ elu. Sives molecular, nke a makwaara dị ka gel filtration. Obere protein na-abanye na pores ma nọrọ na ha ogologo oge. Nnukwu protein enweghị ike ịbanye na pores wee pụta ozugbo.

5. Gịnị bụ usoro ọcha nkeusoro ọcha nke protein? Ultracentrifugation: Enwere ike iji protein dị ọcha iji chọpụta ịdị arọ molekụla ma nwee ike iji ya dị ka protein. E kewapụrụ nhazi nke protein ndị nwere njupụta dị iche iche.