Oligonucleotide (Oligonucleotide), n'ozuzu na-ezo aka na mpempe polynucleotide linear nke 2-10 nucleotide residues jikọtara site na phosphodiester bonds, ma mgbe a na-eji okwu a, ọnụ ọgụgụ nke nucleotide residues Enweghị iwu siri ike. N'ọtụtụ akwụkwọ, a na-akpọkwa mkpụrụ ndụ polynucleotide nwere 30 ma ọ bụ karịa nucleotide residues oligonucleotides. Enwere ike ịmepụta oligonucleotides na-akpaghị aka site na ngwá ọrụ. Enwere ike iji ha mee ihe dị ka ndị na-emepụta DNA synthesis primers, gene probes, wdg, ma nwee ọtụtụ ihe eji eme nchọpụta ihe omimi nke ihe omimi nke oge a.

ngwa

A na-ejikarị oligonucleotides dị ka nyocha iji chọpụta nhazi nke DNA ma ọ bụ RNA, a na-ejikwa ya na usoro dị ka mgbawa mkpụrụ ndụ ihe nketa, electrophoresis, na fluorescence na ọnọdụ hybridization.

Enwere ike iji DNA mebere oligonucleotide na mmeghachi omume polymerization, nke nwere ike ịbawanye ma kwado ihe fọrọ nke nta ka ọ bụrụ iberibe DNA niile. N'ime usoro a, a na-eji oligonucleotide mee ihe dị ka primer iji jikọta ya na mpempe nkwado akpọrọ na DNA iji mee DNA. .

A na-eji oligonucleotides na-achịkwa iji gbochie iberibe RNA ma gbochie ịsụgharị ha na protein, nke nwere ike ịrụ ọrụ ụfọdụ n'ịkwụsị ọrụ nke mkpụrụ ndụ kansa.