The isi ụkpụrụ na àgwà nke nucleic acid extractor

Sistemụ mmịpụta nucleic acid (Nucleic Acid Extraction System) bụ ngwa na-eji reagents nucleic acid na-amịpụta reagents iji mezue mmịpụta nucleic acid ozugbo. A na-eji ya eme ihe na ebe a na-ahụ maka ọrịa, nchọpụta ọrịa ụlọ ọgwụ, nchekwa mmịnye ọbara, nchọpụta nyocha, nyocha microbial gburugburu ebe obibi, nyocha nchekwa nri, ịzụ anụmanụ na nyocha ihe ọmụmụ ihe omimi na mpaghara ndị ọzọ.
1. Usoro mmiri mmiri, nke a makwaara dị ka usoro pipetting, bụ iji wepụ nucleic acid site n'ime ka ọkpụkpụ magnetik na-ebufe mmiri mmiri. N'ozuzu, a na-enweta nnyefe ahụ site na ịchịkwa ogwe aka robot site na sistemụ arụmọrụ. Usoro mmịpụta bụ nke a:

6c1e1c0510-300x300 Sayensị ndụ BM, ihe nzacha maka ndụmọdụ Pipette

1) Lysis: Tinye lysis ngwọta na sample, na-aghọta na Ịgwakọta na zuru mmeghachi omume nke mmeghachi omume ngwọta site n'ibu ije na kpo oku, mkpụrụ ndụ na-lysed, na nucleic acid tọhapụrụ.

2) Adsorption: Tinye bead magnetik na lysate sample, gwakọta nke ọma, ma jiri bead magnet nwee njikọ siri ike maka acid nucleic n'okpuru nnu dị elu na pH dị ala iji gbasaa acid nucleic. N'okpuru ọrụ nke oghere ndọta dị n'èzí, a na-ekewa beads magnet na ngwọta. , jiri ọnụ ọnụ wepụ mmiri mmiri ma tụba ya na tankị mkpofu, ma tụfuo ọnụ.

3) Ịsacha: Wepu oghere ndọta dị n'èzí, dochie ya na ọnụ ọhụrụ ma gbakwunye ebe nchekwa ịsa ahụ, jikọta nke ọma iji wepụ ihe ndị na-adịghị ọcha, ma wepụ mmiri mmiri n'okpuru ọrụ nke oghere magnetik.

4) Elution: Wepu oghere ndọta dị n'èzí, jiri ọnụ ọhụrụ dochie ya, tinye ihe nchekwa elution, gwakọta nke ọma, wee kewaa acid nucleic agbụ na bead magnet iji nweta acid nucleic dị ọcha.
2. Usoro mmanya ndọta

Usoro mkpara magnetik na-achọpụta nkewa nke acid nucleic site na idozi mmiri mmiri na ịnyefe bead magnet. Ụkpụrụ na usoro bụ otu ihe ahụ dị ka usoro nsị, ma ihe dị iche bụ usoro nkewa n'etiti bead magnet na mmiri mmiri. Usoro ihe ndọta magnetik bụ ikewapụ beads magnet na mmiri efu site na mgbasa ozi nke mkpanaka magnet na bead magnet, ma tinye ha n'ime mmiri na-esote iji ghọta mmịpụta nke nucleic acid.


Oge nzipu: Mee-24-2022