SPME nwere isi atọmmịpụtaụdịdị: Direct Ectraction SPME, Headspace SPME na akpụkpọ ahụ SPME echekwara.

1) mmịpụta ozugbo

Na usoro mmịpụta kpọmkwem, eriri quartz na-ekpuchi yammịpụtaA na-etinye ozugbo n'ime matriks nlele, ma a na-ebufe ihe ndị e lekwasịrị anya ozugbo site na matriks sample gaa na ngalaba nkwụsị nke mmịpụta. N'oge a na-arụ ọrụ ụlọ nyocha, a na-ejikarị usoro mkpasu iwe na-eme ka mgbasa nke akụrụngwa nyocha dị ngwa site na matrix sample ruo na nsọtụ nke oge mmịpụta. Maka ihe nlele gas, ikuku ikuku nke gas zuru ezu iji mee ka nha nha nke ihe nyocha dị n'etiti usoro abụọ ahụ. Mana maka ihe nlele mmiri, ọsọ nke ihe ndị dị na mmiri bụ 3-4 iwu nke ịdị ukwuu karịa nke gas, yabụ teknụzụ ngwakọta dị irè dị mkpa iji nweta mgbasa ngwa ngwa nke ngwa n'ime ihe nlele ahụ. The ọzọ-eji Ịgwakọta usoro na-agụnye: speeding elu sample eruba ọnụego, n'ikwe nke mmịpụta eriri isi ma ọ bụ sample akpa, rotor edemede na ultrasound.

N'otu aka ahụ, usoro ndị a na-agwakọta usoro na-eme ka ọnụego mgbasa nke ihe ndị ahụ dị na nnukwu matrix sample, na n'aka nke ọzọ, belata mmetụta a na-akpọ "mpaghara ọnwụ" kpatara oyi akwa nke mkpuchi mkpuchi ihe nkiri nke e guzobere na ya. mgbidi dị n'èzí nke oge nkwụsị nke mmịpụta.

2) Mwepu isi

Na ọnọdụ mmịpụta headspace, usoro mmịpụta nwere ike kewaa ụzọ abụọ:
1. Akụkụ a nyochara na-agbasa ma na-abanye n'ime mmiri mmiri ruo oge gas;
2. A na-ebufe akụkụ a nyochara site na gas gas na oge nkwụsị nke mmịpụta.
Mgbanwe a nwere ike igbochi oge mmịpụta ahụ site na ihe ndị dị elu na ihe ndị na-adịghị agbanwe agbanwe na ụfọdụ matrices sample (dị ka nzuzo mmadụ ma ọ bụ mmamịrị). Na usoro mmịpụta a, ọsọ mmịpụta nke nzọụkwụ 2 na-adịkarị ukwuu karịa ọsọ mgbasa nke nzọụkwụ 1, yabụ nzọụkwụ 1 na-aghọ usoro nchịkwa nke mmịpụta. Ya mere, ihe ndị na-agbanwe agbanwe nwere ọnụego mmịpụta ngwa ngwa karịa ihe ndị na-adịghị agbanwe agbanwe. N'ezie, maka ihe ndị na-agbanwe agbanwe, n'okpuru otu ọnọdụ ngwakọta ihe atụ, oge nhata nke mmịpụta isi dị mkpụmkpụ karịa nke mmịpụta ozugbo.

3) Mwepụta nchebe nke ụbụrụ

Ebumnuche bụ isi nke nchebe akpụkpọ ahụ SPME bụ iji chebe ihemmịpụtaOge nkwụsị site na mmebi mgbe ị na-enyocha ihe nlele ruru unyi. E jiri ya tụnyere isi SPME mmịpụta, usoro a bara uru karịa maka mmịpụta na ịba ụba nke akụrụngwa siri ike na-agbanwe agbanwe. Tụkwasị na nke ahụ, ihe nkiri nchebe nke ihe ndị pụrụ iche mere na-enye ụfọdụ nhọrọ nhọrọ maka usoro mmịpụta.