Oligonucleotide (Oligonucleotide), feem ntau yog hais txog ib feem ntawm cov kab polynucleotide ntawm 2-10 nucleotide residues txuas los ntawm phosphodiester bonds, tab sis thaum siv lo lus no, tus naj npawb ntawm nucleotide residues tsis muaj kev cai nruj. Hauv ntau cov ntaub ntawv, polynucleotide molecules uas muaj 30 lossis ntau dua nucleotide residues kuj hu ua oligonucleotides. Oligonucleotides tuaj yeem raug tsim los ntawm cov twj paj nruag. Lawv tuaj yeem siv los ua DNA synthesis primers, gene probes, thiab lwm yam, thiab muaj ntau yam kev siv nyob rau hauv niaj hnub kev tshawb fawb molecular biology.

daim ntawv thov

Oligonucleotides feem ntau yog siv los ua kev sojntsuam los txiav txim cov qauv ntawm DNA lossis RNA, thiab siv rau hauv cov txheej txheem xws li gene chip, electrophoresis, thiab fluorescence hauv situ hybridization.

Cov DNA synthesized los ntawm oligonucleotide tuaj yeem siv rau hauv cov saw hlau polymerization cov tshuaj tiv thaiv, uas tuaj yeem nthuav dav thiab paub meej tias yuav luag txhua qhov DNA tawg. Hauv cov txheej txheem no, oligonucleotide yog siv los ua ib qho primer los ua ke nrog cov ntawv sau ua ke hauv DNA los ua ib daim ntawv theej DNA. .

Regulatory oligonucleotides yog siv los inhibit RNA fragments thiab tiv thaiv lawv los ntawm kev muab txhais ua cov proteins, uas tuaj yeem ua lub luag haujlwm tseem ceeb hauv kev txwv kev ua haujlwm ntawm cov qog nqaij hlav cancer.