Solid Phase Extraction (SPE) yog ib qho qauv ua ntej siv tshuab tsim los txij li xyoo 1980s. Nws yog tsim los ntawm kev sib xyaw ua ke ntawm kua-khoom extraction thiab kua chromatography. Feem ntau yog siv rau kev sib cais, purification thiab enrichment ntawm cov qauv. Lub hom phiaj tseem ceeb yog txo cov qauv matrix cuam tshuam thiab txhim kho kev tshawb pom rhiab heev.

Raws li txoj kev xav ntawm cov kua-khoom chromatography, SPE thev naus laus zis siv cov kev xaiv adsorption thiab xaiv elution los txhawb nqa, cais thiab ntxuav cov qauv. Nws yog cov txheej txheem rho tawm lub cev nrog rau cov theem ua kua thiab khoom; nws tuaj yeem raug kwv yees los ntawm suav tias yog txheej txheem chromatographic yooj yim.
Schematic daim duab ntawm cov khoom theem extraction
SPE yog lub hauv paus ntsiab lus ntawm kev sib cais ntawm cov kua chromatography siv cov kev xaiv adsorption thiab xaiv elution. Txoj kev siv ntau tshaj plaws yog kom dhau cov kua qauv tshuaj los ntawm cov adsorbent, khaws cov tshuaj mus kuaj, thiab tom qab ntawd siv cov kuab tshuaj uas tsim nyog los yaug tawm cov impurities, thiab tom qab ntawd maj mam muab cov tshuaj mus kuaj nrog me me. hnyav, thiaj li ua tiav lub hom phiaj ntawm kev sib cais sai, purification thiab concentration. Nws kuj tseem tuaj yeem xaiv adsorb cuam tshuam impurities thiab cia cov tshuaj ntsuas ntws tawm; los yog kom adsorb impurities thiab ntsuas cov khoom nyob rau tib lub sijhawm, thiab tom qab ntawd siv cov kuab tshuaj tsim nyog los xaiv cov khoom ntsuas.
Cov extractant ntawm cov khoom-theem extraction txoj kev yog cov khoom, thiab nws txoj kev ua hauj lwm yog raws li qhov tseeb hais tias cov khoom yuav tsum tau ntsuas thiab cov coexisting interfering Cheebtsam nyob rau hauv cov qauv dej muaj sib txawv zog ntawm cov khoom-theem extraction tus neeg saib xyuas, kom lawv. sib cais los ntawm ib leeg. Cov txheej txheem tshem tawm cov khoom yog ib qho tshwj xeeb muab tub lim uas muaj C18 lossis C8, nitrile, amino thiab lwm pawg.