Nucleic Acid Extraction System (Nucleic Acid Extraction System) yog ib qho cuab yeej siv sib txuam nucleic acid extraction reagents kom ua tiav cov qauv nucleic acid extraction. Dav siv nyob rau hauv cov chaw tswj kab mob, kuaj kab mob, kuaj ntshav kev nyab xeeb, forensic identification, ib puag ncig microbial kuaj, kuaj zaub mov kev nyab xeeb, tsiaj txhu thiab molecular biology tshawb fawb thiab lwm yam.
1. Txoj kev nqus, tseem hu ua pipetting method, yog kom rho tawm nucleic acid los ntawm immobilizing magnetic hlaws dai thiab hloov cov kua. Feem ntau, kev hloov pauv tau pom tau los ntawm kev tswj cov caj npab neeg hlau los ntawm kev ua haujlwm. Txoj kev extraction yog raws li nram no:

1) Lysis: Ntxiv cov tshuaj lysis rau cov qauv, thiab paub txog kev sib xyaw thiab tag nrho cov tshuaj tiv thaiv cov tshuaj tiv thaiv los ntawm kev siv tshuab thiab cua sov, lub hlwb raug lysed, thiab cov nucleic acid raug tso tawm.

2) Adsorption: Ntxiv cov hlaws sib nqus rau cov qauv lysate, sib tov kom huv si, thiab siv cov hlaws sib nqus kom muaj kev sib raug zoo rau cov nucleic acids hauv qab ntsev thiab pH tsawg rau adsorb nucleic acids. Nyob rau hauv qhov kev txiav txim ntawm ib qho chaw sib nqus sab nraud, cov hlaws sib nqus tau sib cais los ntawm kev daws teeb meem. , siv lub taub hau tshem tawm cov kua thiab muab pov tseg rau hauv lub tank pov tseg, thiab muab pov tseg lub taub hau.

3) Ntxuav: Tshem tawm cov hlau nplaum sab nraud, hloov nrog cov lus qhia tshiab thiab ntxiv cov ntxhua khaub ncaws, sib tov zoo kom tshem tawm cov impurities, thiab tshem tawm cov kua hauv qab qhov kev txiav txim ntawm sab nraud magnetic teb.

4) Elution: Tshem tawm sab nraud magnetic teb, hloov nrog lub tswv yim tshiab, ntxiv elution tsis, sib tov zoo, thiab ces cais cov bound nucleic acid los ntawm magnetic hlaws dai kom tau purified nucleic acid.
2. Magnetic bar txoj kev

Txoj kev sib nqus pas nrig paub txog kev sib cais ntawm nucleic acids los ntawm kev kho cov kua thiab hloov cov hlaws sib nqus. Lub hauv paus ntsiab lus thiab txheej txheem yog tib yam li cov txheej txheem suction, tab sis qhov txawv yog txoj kev sib cais ntawm cov hlaws sib nqus thiab cov kua. Txoj kev sib nqus bar yog cais cov hlaws sib nqus los ntawm cov kua khib nyiab los ntawm adsorption ntawm cov hlau nplaum mus rau cov hlaws sib nqus, thiab muab tso rau hauv cov kua tom ntej kom paub txog kev rho tawm ntawm nucleic acid.