Kev sib cais thiab kev ua kom huv ntawm cov protein yog dav siv hauv kev tshawb fawb biochemistry thiab daim ntawv thov thiab yog ib qho tseem ceeb ntawm kev ua haujlwm. SCG Protein Purification System Lub Tuam Txhab-Saipu Instrument tau sau cov kev sib cais tsis zoo thiab cov ntsiab lus sib cais zoo ntawmprotein ntaupurification rau sawv daws. Ib qho eukaryotic cell uas muaj peev xwm muaj ntau txhiab cov proteins sib txawv, qee qhov muaj nplua nuj heev thiab qee qhov tsuas muaj ob peb daim ntawv. Txhawm rau kawm qee yam protein, nws yog ib qho tsim nyog yuav tsum ua ntej ntxuav cov protein los ntawm lwm cov proteins thiab cov tsis muaj protein ntau.

Kev sib cais

Thaum cov protein extract (qee zaum sib xyaw nrog nucleic acids, polysaccharides, thiab lwm yam) tau txais, cov txheej txheem tsim nyog raug xaiv los cais qhov xav tau.protein ntaulos ntawm lwm yam impurities. Feem ntau, cov kauj ruam ntawm kev sib cais no siv txoj hauv kev xws li salting tawm, isoelectric point accumululation thiab organic solvent fractionation. Cov txheej txheem no yog tus cwj pwm los ntawm kev yooj yim thiab kev ua haujlwm loj, uas tuaj yeem tshem tawm ntau yam impurities thiab mloog zoo rau cov kua protein. Qee cov protein extracts yog loj nyob rau hauv ntim thiab tsis haum rau concentration los ntawm tsub zuj zuj los yog salting tawm. Koj tuaj yeem xaiv ultrafiltration, gel pom, khov lub tshuab nqus tsev ziab lossis lwm txoj hauv kev rau kev xav.

Kev sib cais zoo

Tom qab qhov ntxhib feem ntawm cov qauv, lub ntim feem ntau me me, thiab feem ntau ntawm cov impurities tau raug tshem tawm. Rau kev ua kom huv ntxiv, cov txheej txheem chromatography feem ntau suav nrog gel filtration, ion exchange chromatography, adsorption chromatography, thiab affinity chromatography. Yog tias tsim nyog, koj tuaj yeem xaiv electrophoresis, suav nrog cheeb tsam electrophoresis, isoelectric point set, thiab lwm yam raws li cov txheej txheem purification zaum kawg. Txoj kev siv rau kev sib cais theem sib cais feem ntau me me hauv kev npaj, tab sis muaj kev daws teeb meem siab.

Crystallization yog txheej txheem kawg ntawm kev sib cais ntawm cov protein thiab purification. Txawm hais tias cov txheej txheem crystallization tsis ua kom ntseeg tau tias cov protein yuav tsum zoo ib yam, nws tsuas yog thaum qee cov protein muaj txiaj ntsig zoo hauv kev daws teeb meem los tsim cov khoom siv lead ua. Cov txheej txheem crystallization nws tus kheej yog nrog los ntawm qee qib ntawm kev ua kom huv, thiab recrystallization tuaj yeem tshem tawm me me ntawm cov protein uas tsis zoo. Txij li thaum denaturedprotein ntautsis tau pom thaum lub sij hawm crystallization txheej txheem, protein crystallization tsis yog tsuas yog ib tug kos npe rau ntawm purity, tab sis kuj yog ib tug muaj zog cov lus qhia los txiav txim tias cov khoom nyob rau hauv nws lub ntuj lub xeev.