Cov txheej txheem dav dav rau kev rho tawm cov theem yog raws li hauv qab no:

1. Ua kom cov adsorbent: Yaug cov txheej txheem tshem tawm cov khoom siv nrog cov kuab tshuaj tsim nyog ua ntej muab cov qauv kom cov adsorbent ntub dej, uas tuaj yeem nqus cov hom phiaj sib txuas lossis cuam tshuam cov khoom sib txuas. Cov qauv sib txawv ntawm cov txheej txheem tshem tawm cov cartridge ua kom siv cov kuab tshuaj sib txawv:

(1) Tsis muaj zog polar lossis non-polar adsorbents siv nyob rau hauv reversed-phase solid-phase extraction feem ntau yog rinsed nrog ib tug dej-soluble organic hnyav, xws li methanol, thiab ces yaug nrog dej los yog tsis. Nws kuj tseem tuaj yeem yaug nrog cov kuab tshuaj muaj zog (xws li hexane) ua ntej yaug nrog methanol kom tshem tawm cov impurities adsorbed ntawm cov adsorbent thiab lawv cuam tshuam nrog lub hom phiaj.

(2) Lub ncov qaumteb qabteb adsorbent siv nyob rau hauv ib txwm-theem khoom-theem rho tawm feem ntau yog eluted nrog cov organic hnyav (cov qauv matrix) qhov twg lub hom phiaj compound nyob.

(3) Cov adsorbent siv nyob rau hauv ion-pauv khoom theem rho tawm tuaj yeem ntxuav nrog cov qauv hnyav thaum nws siv rau cov qauv hauv cov kuab tshuaj tsis muaj polar organic; Thaum nws yog siv rau cov qauv hauv cov kuab tshuaj polar, nws tuaj yeem ntxuav nrog dej-soluble organic solvents Tom qab ntxuav, yaug nrog cov tshuaj aqueous ntawm tus nqi pH thiab muaj qee cov organic solvents thiab ntsev.

Txhawm rau kom cov sorbent hauv SPE cartridge ntub dej tom qab ua kom tiav thiab ua ntej ua piv txwv ntxiv, txog 1 ml ntawm cov kuab tshuaj rau kev ua kom muaj zog yuav tsum tau khaws cia rau ntawm sorbent tom qab ua kom tiav.

2. Sample loading: Ncuav cov kua los yog yaj cov qauv mus rau hauv lub tshuab nqus tsev twj tso kua mis, thiab tom qab ntawd siv lub tshuab nqus tsev, siab los yog centrifugation kom cov qauv nkag mus rau hauv lub adsorbent.

3. Ntxuav thiab elution: Tom qab cov qauv nkag mus rau hauv lub adsorbent thiab lub hom phiaj compound yog adsorbed, lub weakly retained interfering compound yuav tsum tau ntxuav nrog ib tug weaker hnyav, thiab ces lub hom phiaj compound yuav eluted nrog ib tug muaj zog hnyav hnyav thiab sau. . Yaug thiab Elution Raws li tau piav qhia dhau los, eluent lossis eluent tuaj yeem dhau los ntawm cov adsorbent los ntawm lub tshuab nqus tsev, siab lossis centrifugation.

Yog tias cov adsorbent raug xaiv kom muaj qhov tsis muaj zog lossis tsis muaj adsorption rau lub hom phiaj sib xyaw thiab muaj zog adsorption rau qhov cuam tshuam cov khoom sib txuas, lub hom phiaj tseem tuaj yeem muab yaug thiab sau ua ntej, thaum cov khoom cuam tshuam tau khaws cia (adsorption). ) ntawm cov adsorbent, ob qhov sib cais. Nyob rau hauv feem ntau, lub hom phiaj compound yog khaws cia nyob rau hauv lub adsorbent, thiab thaum kawg eluted nrog ib tug muaj zog hnyav, uas yog ntau conducive rau lub purification ntawm tus qauv.