Kev kuaj nucleic acid tiag tiag yog txhawm rau txheeb xyuas seb puas muaj nucleic acid (RNA) ntawm tus kabmob tshiab no hauv lub cev ntawm qhov kev xeem. Cov nucleic acid ntawm txhua tus kab mob muaj ribonucleotides, thiab tus naj npawb thiab kev txiav txim ntawm ribonucleotides muaj nyob rau hauv cov kab mob sib txawv, ua rau txhua tus kab mob tshwj xeeb.
Lub nucleic acid ntawm tus kabmob tshiab no kuj yog qhov tshwj xeeb, thiab kev kuaj pom nucleic acid yog qhov tshwj xeeb ntawm kev kuaj pom tus kabmob nucleic acid ntawm tus kabmob tshiab. Ua ntej kuaj nucleic acid, nws yuav tsum tau sau cov qauv ntawm tus neeg mob cov hnoos qeev, caj pas swab, bronchoalveolar lavage kua, ntshav, thiab lwm yam, thiab los ntawm kev ntsuam xyuas cov qauv no, nws tuaj yeem pom tias tus neeg mob lub ntsws muaj kab mob. Kev kuaj pom tus mob coronavirus tshiab nucleic acid feem ntau yog siv rau kev kuaj mob caj pas. Cov qauv no tau muab cais thiab ua kom huv, thiab qhov ua tau tshiab tus mob coronavirus nucleic acid tau muab rho tawm los ntawm nws, thiab cov kev npaj rau kev sim tau npaj txhij.

Kev kuaj pom tus mob coronavirus tshiab nucleic acid feem ntau yog siv fluorescence quantitative RT-PCR thev naus laus zis, uas yog kev sib xyaw ntawm fluorescence quantitative PCR technology thiab RT-PCR thev naus laus zis. Hauv cov txheej txheem tshawb nrhiav, RT-PCR thev naus laus zis yog siv los thim rov qab cov ntaub ntawv nucleic acid (RNA) ntawm tus kabmob tshiab no rau hauv cov khoom siv deoxyribonucleic acid (DNA); Tom qab ntawd fluorescence quantitative PCR thev naus laus zis yog siv los rov ua cov DNA tau los ntawm ntau qhov ntau. DNA replicated yog kuaj thiab sau npe nrog kev soj ntsuam kev sib deev. Yog tias muaj tus kab mob coronavirus tshiab nucleic acid, lub cuab yeej tuaj yeem kuaj pom cov teeb liab fluorescent, thiab, raws li DNA txuas ntxiv rov ua dua, cov teeb liab fluorescent tseem nce ntxiv, yog li tsis ncaj qha kuaj pom muaj tus kabmob tshiab.