Hoʻohana nui ʻia ka hoʻokaʻawale ʻana a me ka hoʻomaʻemaʻe ʻana o nā protein i ka noiʻi biochemistry a me ka noi ʻana a he mākaukau hana koʻikoʻi. ʻO SCG Protein Purification System Company-Saipu Instrument i hōʻuluʻulu i ka hoʻokaʻawale ʻana a me ka ʻike hoʻokaʻawale maikaʻi opūmuaka hoomaemae ana no na mea a pau. Hiki i kekahi cell eukaryotic maʻamau ke loaʻa nā kaukani o nā protein ʻokoʻa, waiwai nui kekahi a he mau kope wale nō kekahi. No ke aʻo ʻana i kekahi protein, pono e hoʻomaʻemaʻe mua i ka protein mai nā protein ʻē aʻe a me nā molekole non-protein.

Hoʻokaʻawale koʻikoʻi

I ka loaʻa ʻana o ka extract protein (i hui pū ʻia me ka nucleic acids, polysaccharides, etc.), koho ʻia kahi ʻano kūpono e hoʻokaʻawale i ka makemake.pūmuamai nā haumia ʻē aʻe. ʻO ka maʻamau, hoʻohana kēia ʻanuʻu o ka hoʻokaʻawale ʻana i nā ʻano e like me ka paʻakai ʻana, ka hōʻiliʻili ʻana o ka isoelectric a me ka haʻihaʻi solvent organik. Hōʻike ʻia kēia mau ʻano e ka maʻalahi a me ka hiki ke hana nui, hiki ke wehe i nā mea haumia he nui a hoʻopaʻa i ka hopena protein. Nui ka leo o kekahi mau ʻāpana protein a ʻaʻole kūpono i ka noʻonoʻo ʻana ma ka hōʻiliʻili ʻana a i ʻole ka paʻakai ʻana. Hiki iā ʻoe ke koho i ka ultrafiltration, ka kānana gel, ka maloʻo maloʻo maloʻo a i ʻole nā ​​​​ʻano hana ʻē aʻe no ka noʻonoʻo.

Hoʻokaʻawale maikaʻi

Ma hope o ka haʻihaʻi ʻana o ka hāpana, liʻiliʻi ka leo, a ua wehe ʻia ka hapa nui o nā haumia. No ka hoʻomaʻemaʻe hou aku, ʻo nā ʻano chromatography ka mea maʻamau i ka kānana gel, ka chromatography hoʻololi ion, ka chromatography adsorption, a me ka chromatography affinity. Inā pono, hiki iā ʻoe ke koho i ka electrophoresis, me ka electrophoresis zone, kahi hoʻonohonoho isoelectric, a me nā mea ʻē aʻe e like me ke kaʻina hoʻomaʻemaʻe hope. ʻO ke ala i hoʻohana ʻia no ka hoʻokaʻawale ʻana i ka pae ʻāpana ʻāpana liʻiliʻi ma ka hoʻolālā ʻana, akā me ka hoʻonā kiʻekiʻe.

ʻO ka crystallization ka hana hope loa o ka hoʻokaʻawale ʻana a me ka hoʻomaʻemaʻe. ʻOiai ʻaʻole hōʻoia ke kaʻina hana crystallization e pono ke ʻano like ʻole ka protein, ʻo ia wale nō ke loaʻa ka pono o kekahi protein i ka hopena e hana i kahi aniani. Hoʻopili pū ʻia ke kaʻina hana crystallization me kahi pae o ka hoʻomaʻemaʻe ʻana, a hiki i ka recrystallization ke wehe i kahi liʻiliʻi o ka protein adulterated. Mai ka denaturedpūmuaʻAʻole i ʻike ʻia i ka wā o ka crystallization, ʻo ka crystallization protein ʻaʻole ia he hōʻailona o ka maʻemaʻe, akā he alakaʻi ikaika hoʻi e hoʻoholo ai aia ka huahana i kona kūlana kūlohelohe.