Nā hana ohoʻomaʻemaʻe protein:

ʻO ke ʻano o ka hoʻomaʻemaʻe ʻana i ka protein, ka hoʻokaʻawale ʻana a me ka hoʻomaʻemaʻe ʻana i ka protein, hoʻokuʻu ʻia ka protein mai nā ʻāpana kumu a i ʻole nā ​​ʻiʻo i loko o kahi moku i hoʻoheheʻe ʻia a noho mau i ke ʻano kūlohelohe maoli me ka ʻole o ka nalowale o ka hana biological. No kēia kumu, pono ka mea e hoʻoneʻe i ka ʻiʻo holoholona a me ka adipose, ʻo ka mea hua e ʻili i ka haumia o ka ʻaʻahu hua mai nā mea e like me nā tannins, a ʻoi aku ka maikaʻi o ka hua aila e hoʻohana i kahi mea hoʻoheheʻe haʻahaʻa haʻahaʻa.

1. ʻO ke ʻano o ka hoʻomaʻemaʻe ʻana i ka protein, ka nui a me nā ʻano hoʻokaʻawale hoʻokaʻawale ʻē aʻe: hoʻohana ka dialysis a me ka ultrafiltration i kēia mau protein e hele i ke ʻano o nā moleke hiki ʻole ke hele i ka membrane semi-permeable; ʻoi aku ka nui o ka hopena o ka maikaʻi a me ka nui o ka protein i loko o ka waena. ʻOi aku ka wikiwiki o ka hoʻoponopono ʻana; ʻO ka kānana gel he ʻano chromatography kolamu.

2. Hoʻokaʻawale i ka ʻokoʻa solubility i ka hoʻohana ʻana: electro-precipitation no ka mea ʻo ka ʻupena ʻupena o nā molekele protein aia ma kahi helu isoelectric, e hōʻemi ana i ka repulsion electrostatic ma waena o nā molekala, maʻalahi e hōʻuluʻulu, a me ka liʻiliʻi solubility; hoʻohana ʻia ka wai paʻakai me ka wai paʻakai Hoʻonui a hoʻemi paha ka ʻike i ka solubility o ka protein.

3. Hoʻokaʻawale ʻia ka uku a hoʻopaʻa ʻia e nā ʻano hana like ʻole, ʻo nā mea nui e pili ana i ka electrophoresis a me ka chromatography hoʻololi ion.

4. Nā hana ohoʻomaʻemaʻe proteinʻO ka adsorption koho a me ka hoʻokaʻawale ʻana o nā protein e hoʻohana i ka ikaika o ka adsorption particle.

5. Hoʻokumu ʻia ka chromatography affinity ma ka hoʻokaʻawale ʻana o nā ligands. ʻO kēia hiʻohiʻona olaola hiki i nā molekala protein ke hoʻopaʻa pono ma mua o ka covalently i kekahi mole i kapa ʻia he ligand.

6. Ke ʻano o ka hoʻomaʻemaʻe ʻana i ka protein: ʻO ke ala haʻahaʻa haʻahaʻa haʻahaʻa haʻahaʻa hoʻoheheʻe wai: E hoʻohana i nā mea hoʻoheheʻe kūlohelohe i hui pū ʻia me ka wai, e like me ka methanol, ethanol, e hōʻemi i ka solubility a me ka ua o ka hapa nui o nā protein. ʻOi aku ka kiʻekiʻe o kēia ʻano ma mua o ka hoʻonā ʻana i ka paʻakai, akā ʻoi aku ka maikaʻi o ka protein i ka wā maʻalahi, pono e hana ʻia ma ka wela haʻahaʻa.

Pehea e hoʻomaʻemaʻe aihoʻomaʻemaʻe protein:

Pehea e hoʻomaʻemaʻe ai i ka protein, ʻo ka protein kahi mole nui, a me nā ʻano nui o ka albumin, aia kekahi mau ala maʻalahi e hoʻokaʻawale i ka protein a me nā mea liʻiliʻi liʻiliʻi, a me ka hui pū ʻana o ka protein. ʻO nā ala nui no ka hoʻokaʻawale ʻana i nā protein o nā nui molekala like ʻole he dialysis, ultrafiltration, gel filtration, a me centrifugal separation. Hoʻohana pinepine ʻia ka dialysis a me ka ultrafiltration i nā kaʻina hoʻokaʻawale protein. Hoʻokomo ʻia ka hui dialysis e hoʻokaʻawale ʻia i loko o kahi ʻeke dialysis i hana ʻia me ka membrane semipermeable, a laila hoʻokomo ʻia i loko o ka hopena dialysis no ka hoʻokaʻawale ʻana. ʻO ka Ultrafiltration ke kaʻina o ka hoʻoheheʻe ʻana i ka wai a me nā molekole liʻiliʻi ʻē aʻe me ka hoʻohana ʻana i ka ikaika centrifugal a i ʻole ke kaomi ʻana i loko o kahi membrane semi-permeable a hoʻopaʻa i nā protein i loko o ka membrane semi-permeable. Hiki ke hoʻokaʻawale ʻia kēia mau ʻano ʻelua mai nā macromolecules i hoʻokumu ʻia i ka protein a me nā molekole liʻiliʻi paʻakai. Hoʻohana pinepine ʻia lākou i ka paʻakai, hoʻohana pū ʻia me ke ʻano hoʻoheheʻe paʻakai, a i ʻole hiki ke hoʻolauna ʻia e lawe i kēia mau ʻano ʻelua ma hope o ka paʻakai ʻana me ka hoʻohana ʻana i nā wai paʻakai inorganic.

1. Pehea e hoʻomaʻemaʻe ai i ka protein ma ke ʻano paʻakai: ʻO ke kumu o ka paʻakai paʻakai ʻo ia ka hoʻoheheʻe ʻia o ka protein i loko o ka wai paʻakai dilute e piʻi aʻe me ka piʻi ʻana o ka paʻakai, akā ke piʻi ka paʻakai i kekahi waiwai, e hoʻonui ʻia ka hana wai. ʻO ka emi ʻana, ʻo ia ka mea e hiki ai i kekahi mau protein ke hoʻohua i nā koina ma o ka ʻili o ka molecular structure e hoʻoneʻe mālie ʻia, a hoʻopau mālie ʻia ka membrane hydration, a hiki i ka hopena ke hoʻoulu i nā protein a me nā molekala e pili ana i ka agglomeration a hoʻoheheʻe ʻia mai nā hopena like ʻole.

2. ʻO ke ʻano o ka hoʻoheheʻe ʻana i ka wai: ʻElua kumu e hōʻemi ai i ka solubility o ka protein i loko o nā mea hoʻoheheʻe organik: pehea e hoʻomaʻemaʻe ai i ka protein ma ke ʻano he paʻakai me ka wai maloʻo; ʻO ka lua, hiki ke hoʻemi ʻia ka dielectric mau o nā mea hoʻoheheʻe organik ma mua o ka wai.

3. ʻO ka mea hoʻoheheʻe protein: hana wale ka protein ma ke ʻano hoʻokahi a i ʻole kekahi o ka ua o ka protein, ʻo nā mea maʻamau he alkaline, protein, lectin, a me nā metala kaumaha.

4. Polyethylene glycol precipitation: Pehea e hoʻomaʻemaʻe i ka wai-soluble non-ionic polymers nohoʻomaʻemaʻe protein, e like me ka polyethylene glycol a me ka sodium dextran sulfate e hoʻokahe i nā protein.