Menene hanyar tsarkakewa natsarin tsarkakewa sunadaran? Wajibi ne a san jerin DNA na furotin da aka tsarkake, don ganin waɗanne sel ko kyallen takarda sun fi ƙarfin gaske a cikin kwayar halittar da aka yi niyya, da kuma tsara abubuwan da ake amfani da su don ƙara girman guntun DNA ɗin da aka yi niyya. Wannan shine abin da ake kira sayan gutsuttsun kwayoyin halitta.

Gina vector na magana: A cikin prokaryotic ko eukaryotic magana vector tare da halayen da aka samu ta hanyar magana da kansa, babban matsalar wannan mataki shine gina plasmid da kwayoyin sha'awa, da tsarin magana. Lokacin magana na prokaryotic gajere ne, farashin yana da ƙasa, kuma babban adadin magana shine fifiko; Ba a bayyana kwayar halitta a cikin E. coli ba, kuma matsalar tana faruwa a cikin ingantawar codon. Dangane da mafi kyawun aiki da tsarkakewa na furotin, masu bincike sun zaɓi bayyana a cikin yisti Pichi. Nasarar magana na inganta codon yana da mahimmanci.

Menene hanyar tsarkakewa na tsarin tsarkakewar furotin:

1. Hazo.

2. Electrophoresis: Protein da aka caje yana da girma ko ƙasa da ma'aunin isoelectric kuma ana iya motsa shi zuwa ga madaidaicin lantarki ko ingantaccen lantarki na filin lantarki a cikin filin lantarki. Goyon bayan fim electrophoresis, electrophoresis, da dai sauransu.

3. Dialysis: Hanyar da ke amfani da buhunan dialysis guda biyu don raba manyan kwayoyin halitta daga sunadarai da ƙananan kwayoyin halitta.

4. Chromatography: Ion musayar chromatography yana amfani da kaddarorin sunadaran kyauta. A ƙarƙashin takamaiman pH, caji da kaddarorin sunadaran sun bambanta, kuma ana iya raba su ta hanyar chromatography musayar ion. A cikin chromatography musayar anion, sunadaran sunadaran da ke da ƙarancin iko sun fara ɓarna. Molecular sieves, kuma aka sani da gel tacewa. Ƙananan sunadaran suna shiga cikin pores kuma su zauna a cikin su na dogon lokaci. Manya-manyan sunadaran ba za su iya shiga ramuka ba kuma su fita kai tsaye.

5. Menene hanyar tsarkakewa natsarin tsarkakewa sunadaran? Ultracentrifugation: Ana iya amfani da tsarkakewar furotin don ƙayyade nauyin kwayoyin halitta kuma ana iya amfani dashi azaman furotin. An rabu da samuwar sunadaran da yawa daban-daban.