Oligonucleotides su ne polymers acid nucleic tare da tsararrun jeri na musamman, gami da antisense oligonucleotides (ASOs), siRNAs (ƙananan RNAs masu shiga tsakani), microRNAs, da aptamers. Ana iya amfani da Oligonucleotides don daidaita maganganun kwayoyin halitta ta hanyar matakai daban-daban, gami da RNAi, lalata manufa ta hanyar tsagaitawar RNase H, ƙa'idar rarrabawa, danne RNA mara coding, kunna gene, da tsara tsararrun kwayoyin halitta.

Yawancin oligonucleotides (ASOs, siRNA, da microRNA) suna haɓaka zuwa manufa mRNA ko pre-mRNA ta hanyar haɗin haɗin tushe na haɗin gwiwa, kuma a ka'ida na iya zaɓin daidaita yanayin kowane nau'in kwayar halitta da furotin, gami da da yawa "marasa warkewa" manufa. Aptamers suna da babban alaƙa ga furotin da aka yi niyya, kama da tsarin na uku na ƙwayoyin rigakafi, ba jerin ba. Oligonucleotides kuma yana ba da wasu fa'idodi, gami da ingantaccen samarwa da dabarun shirye-shirye, gajeriyar zagayowar ci gaba, da tasirin dorewa.

Idan aka kwatanta da masu hana ƙananan ƙwayoyin ƙwayoyin cuta na gargajiya, amfani da oligonucleotides a matsayin magunguna wata hanya ce ta asali. Ƙimar oligonucleotides a cikin madaidaicin kwayoyin halitta ya haɓaka sha'awar aikace-aikacen warkewa a cikin ciwon daji, cututtukan zuciya, da cututtuka masu wuya. Amincewa da FDA na kwanan nan don Givosiran, Lumasiran da Viltolarsen sun kawo RNAi, ko hanyoyin jiyya na RNA, cikin babban yanayin haɓaka magunguna.