Nucleic Acid System Extraction System (Nucleic Acid Extraction System) kayan aiki ne wanda ke amfani da madaidaitan abubuwan cirewar acid nucleic don kammala fitar da samfurin nucleic acid ta atomatik. An yi amfani da shi sosai a cibiyoyin kula da cututtuka, ganewar cututtukan asibiti, aminci na ƙarin jini, tantancewa na bincike, gwajin ƙwayoyin cuta na muhalli, gwajin lafiyar abinci, kiwo da binciken ilimin halittu da sauran fannoni.
1. Hanyar tsotsa, wanda kuma aka sani da hanyar pipetting, shine a fitar da acid nucleic ta hanyar hana beads na maganadisu da canja wurin ruwa. Gabaɗaya, ana samun canja wurin ta hanyar sarrafa hannun mutum-mutumi ta tsarin aiki. Tsarin hakar shine kamar haka:

1) Lysis: Ƙara lysis bayani ga samfurin, kuma gane da hadawa da cikakken dauki na dauki bayani ta hanyar inji motsi da dumama, Kwayoyin suna lysed, da kuma nucleic acid aka saki.

2) Adsorption: Ƙara ƙwanƙolin maganadisu zuwa samfurin lysate, haɗuwa sosai, kuma amfani da beads na Magnetic don samun dangantaka mai karfi ga acid nucleic a ƙarƙashin babban gishiri da ƙananan pH don ƙaddamar da acid nucleic. Ƙarƙashin aikin filin maganadisu na waje, an raba beads na maganadisu daga maganin. , Yi amfani da tip don cire ruwa kuma jefar da shi zuwa tankin sharar gida, kuma jefar da tip.

3) Wankewa: Cire filin maganadisu na waje, maye gurbin da sabon tip kuma ƙara buffer ɗin wanki, haɗuwa da kyau don cire ƙazanta, da cire ruwa a ƙarƙashin aikin filin maganadisu na waje.

4) Elution: Cire filin maganadisu na waje, maye gurbin da sabon tip, ƙara buffer elution, gauraya da kyau, sa'an nan kuma raba acid nucleic da aka ɗaure daga ƙwanƙolin maganadisu don samun tsaftataccen acid nucleic.
2. Hanyar mashaya Magnetic

Hanyar sandar maganadisu tana gane rarrabuwar acid nucleic ta hanyar gyara ruwa da canja wurin beads na maganadisu. Ka'ida da tsari iri ɗaya ne da na hanyar tsotsa, amma bambancin shine hanyar rabuwa tsakanin ƙwanƙolin maganadisu da ruwa. Hanyar mashaya maganadisu ita ce raba ƙwanƙolin maganadisu daga ruwan sharar gida ta hanyar adsorption na sandar maganadisu zuwa igiyoyin maganadisu, sannan a saka su cikin ruwa na gaba don gane hakowar acid nucleic.