Ana amfani da rarrabuwa da tsarkakewar sunadaran sosai a cikin bincike da aikace-aikacen biochemistry kuma muhimmin fasaha ne na aiki. Kamfanin SCG Protein Purification System Company-Saipu Instrument ya tattara danyen mai da abun ciki mai kyau na rabuwa.furotintsarkakewa ga kowa da kowa. Kwayoyin eukaryotic na yau da kullun na iya ƙunsar dubban sunadaran sunadaran, wasu suna da wadata sosai wasu kuma sun ƙunshi 'yan kwafi kaɗan kawai. Don yin nazarin wani nau'in furotin, dole ne a fara tsarkake furotin daga wasu sunadaran da kwayoyin da ba na gina jiki ba.

M rabuwa

Lokacin da aka samo abubuwan gina jiki (wani lokacin haɗe da acid nucleic, polysaccharides, da dai sauransu), an zaɓi tsarin hanyoyin da suka dace don raba abubuwan da ake so.furotindaga sauran kazanta. Gabaɗaya, wannan matakin na rabuwa yana amfani da hanyoyi kamar su fitar da gishiri, tara ma'aunin isoelectric da rarrabuwar kaushi. Wadannan hanyoyin suna da sauƙin sauƙi da babban ƙarfin aiki, wanda zai iya cire ƙazanta da yawa kuma ya mayar da hankali ga maganin furotin. Wasu tsantsar furotin suna da girma cikin girma kuma ba su dace da tattarawa ta hanyar tarawa ko fitar da gishiri ba. Za ka iya zaɓar ultrafiltration, gel tacewa, daskarewa injin bushewa ko wasu hanyoyin don maida hankali.

Kyakkyawan rabuwa

Bayan ƙarancin juzu'i na samfurin, ƙarar gabaɗaya ƙarami ne, kuma an cire yawancin ƙazanta. Don ƙarin tsarkakewa, hanyoyin chromatography gabaɗaya sun haɗa da tace gel, chromatography musayar ion, chromatography adsorption, da chromatography affinity. Idan ya cancanta, Hakanan zaka iya zaɓar electrophoresis, gami da yankin electrophoresis, saiti na isoelectric, da sauransu azaman aikin tsarkakewa na ƙarshe. Hanyar da ake amfani da ita don rabuwa matakin yanki gabaɗaya ƙanƙanta ce a cikin tsarawa, amma tare da babban ƙuduri.

Crystallization shine tsari na ƙarshe na rabuwar furotin da tsarkakewa. Kodayake tsari na crystallization bai tabbatar da cewa sunadaran dole ne su kasance daidai ba, kawai lokacin da wani furotin yana da fa'ida a cikin bayani don samar da crystal. Tsarin crystallization kanta yana tare da wani matakin tsarkakewa, kuma recrystallization na iya cire ƙaramin adadin furotin da aka lalata. Tun denaturedfurotinba a taɓa samun shi ba a lokacin aikin ƙirƙira, crystallization sunadaran ba kawai alamar tsarki ba ne, amma kuma jagora mai ƙarfi don sanin cewa samfurin yana cikin yanayin yanayinsa.