Fumonisins Affinity Chromatography

Ka'idar tsarkakewa na ginshiƙin gano Fumonisins na musamman shine amsawar rigakafi tsakanin antigens. Kwayoyin rigakafin monoclonal waɗanda ke ɗauke da gwajin Fumonisins an gyara su a cikin ƙaƙƙarfan tallafin lokaci na ginshiƙin ganowa. Ana amfani da samfurin da ke ɗauke da Fumonisins don gano ginshiƙi na musamman na Fumonisins, wanda za'a iya haɗa shi tare da antibody don samar da hadadden antigen antibody, wanda sai a wanke da ruwa don zuwa kayan da ake nufi. A ƙarshe, haɓakawa tare da eluent, tattara eluent, yi amfani da HPLC don gano abubuwan da ke cikin Fumonisins.


Cikakken Bayani

Tags samfurin

Fumonisins wani guba ne na fungal da aka samar ta hanyar scythes, a halin yanzu akwai abubuwan da aka sani guda 28, Ɗaya daga cikin binciken da aka fi sani kuma mai zurfi shine FB1. An gano cewa masara da kayayyakinta, kamar abincin dabbobi, suna cikin sauki ta hanyar FB1. FB1 shine mafi ƙarfi a cikin Fumonisins kuma yana da mummunar tasirin guba akan dabbobi da yawa. Bincike ya nuna cewa farin doki na FB1 na iya haifar da laushin cututtukan kwakwalwa, ciwon huhu na huhu, bugu da ƙari, har yanzu yana iya haifar da ciwon daji na hanji da ciwon hanta, ciwon daji na ciki da sauran cututtuka, kiwon dabbobi da kuma lafiyar ɗan adam. Don haka, gwaji. yana da mahimmanci kuma.

Babban jerin B & M toxin gano shafi na musamman shine ganewar immuno-affinity da kuma shafi na musamman na FB1 (FB1) .Wannan shafi na iya zaɓin adsorb da vomatoxin B1 (FB1) a cikin samfurin samfurin, don haka za'a iya yin niyya ga tasirin tsarkakewa na shafi. , kuma ana iya gwada samfurin kai tsaye ta HPLC bayan an tsarkake shafi.

Aikace-aikace:
Masara; ciyarwa; man fetur, da dai sauransu.
Aikace-aikace na yau da kullun:
Ana amfani dashi don tsarkakewa na Fumonisins a ciki
substrates hadaddun da ƙananan iyaka. Ƙididdigar ƙididdiga
na TLC/HPLC/GC/LC-MS/EIA;
Yana iya auna ƙididdiga da ƙididdiga
ragowar adadin Fumonisins a cikin masara, abinci, mai mai
da sauran samfurori

Bayanin oda

Sorbents

Siffar

Ƙayyadaddun bayanai

PC/pk

Cat. No

Fumonisins Gane Cartridge Harsashi 1 ml

25

Saukewa: FB-IAC0001
Fumonisins Gane Cartridge   3 ml

20

Saukewa: FB-IAC0003
Shagon fanko don alaƙar chromatography   1 ml, guda biyu na hydrophilic Frits

100

Saukewa: ACC001
Shagon fanko don alaƙar chromatography   3ml, guda biyu na hydrophilic Frits

50

Saukewa: ACC003

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