overview:

C18W (unsealed) is an unsealed reversed PHASE C18 extraction column based on silica gel, with more silanol functional groups on the surface providing additional polar interactions. Extraction of nonpolar compounds by hydrophobicity. At the same time, the activity of silanol group makes it better than C18 to retain some pharmaceutical metabolites and basic compounds, so its retention mechanism includes moderate non-polar and polar secondary effects. The column is reserved for most organic compounds and has a wide range of selectivity. It is a universal stationary phase for the extraction of polar and non-polar compounds.
The column is equivalent to Aglient Accu Bond C18, Bond Elute C18 OH.

details

Matrix: silica gel
Functional group: octadecyl unsealed, silanol functional group
Mechanism of action: reverse phase extraction
Carbon content: 18%
Size: 40-75 microns
Surface area: 300m2/g
Average aperture: 60
Application: soil; Water; Body fluids (plasma/urine, etc.); Food; drug
Typical applications: separation of lipids, ganglioside separation of JPMHW and THE United States CDFA official method: pesticides in food natural products
AOAC method: pigment and sugar analysis in food, drug and metabolite in blood, plasma and urine, protein, DNA macromolecule sample desalting, enrichment of organic matter in environmental water sample, organic acid extraction in beverage, as follows: Extraction and purification of antibiotics, barbiturates, phthalazines, caffeine, drugs, dyes, aromatic oils, fat-soluble vitamins, fungicides, weeding agents, pesticides, carbohydrates, p-hydroxytoluene ester, phenol, phthalate esters, steroids, surfactants, theophylline, etc.

Sorbent Information

Matrix：Silica Functional Group：Unsealed end Octadecyl，Silicon alcohol-based Mechanism of Action：Reversed-phase(RP) extraction Carbon Content：18% Particle Size：45-75μm Surface Area：300m2/g Average Pore Size：60Å

Application

Soil；Water; Body Fluids（plasma/urine etc.）；Food；Medicine

Typical Applications

Separation of lipids and lipids Official methods of Japan’s JPMHW and us CDFA：pesticides in food Natural products The AOAC method：food, sugar,pigment in the blood,plasma,drug and its metabolites in urine protein, DNA samples of macromolecular desalination, the organic matter enrichment in environmental water samples, drinks containing organic acid extraction. Specific example：antibiotics,barbiturates, phthalazine,caffeine,drugs,dyes, aromatic oils, fat-soluble vitamins,fungicides,weeding agents,pesticides,carbohydrates,The extraction and purification of hydroxytoluene,phenol,phthalate,steroid,surfactant and theophylline